■大蒜皮(GAS)已被证明可以改善育肥绵羊的生长性能。然而,GAS影响育肥绵羊的机制尚不清楚。本研究的目的是探讨饲料中添加GAS对生长性能的影响。瘤胃和粪便微生物群,血清和尿液代谢,育肥绵羊瘤胃上皮细胞的转录组学。
■将80g/kg干物质(DM)的GAS添加到育肥绵羊的日粮中,以研究GAS对肠道微生物群的影响,血清和尿液代谢,和育肥绵羊瘤胃上皮组织的转录组。十二只湖羊(体重;BW,23.0±2.3kg,年龄120±3.5d)随机分为两组。CON组为基础饮食,而GAS组在基础日粮中补充GAS。试验期为10周,前两周是预审期。
■GAS组育肥羊的日平均增重明显高于CON组(p<0.05),GAS组育肥羊血清GSH-Px显著升高,MDA显著降低(p<0.05)。根据属的分类水平,在饮食中添加大蒜皮改变了肠道微生物组成,相对丰度被Metanobrevebater显著上调(p<0.05),虽然被Akkermansia显著下调,Parasutterilla,和Guggenheimella(p<0.05)。代谢组学分析发现,GAS组和CON组之间血清中有166种明显不同的代谢产物,尿液中有68种明显不同的代谢产物(p<0.05)。GAS对氨基酸代谢有影响,嘧啶代谢,甲烷代谢,核黄素代谢,和不饱和脂肪酸合成途径(p<0.05)。转录组测序显示差异表达基因主要富集在免疫调节功能,改善育肥羊的健康。
■添加GAS可以通过改变肠道菌群来改善育肥羊的能量代谢和免疫功能,代谢组,和转录组,从而提高育肥羊的生长性能。
UNASSIGNED: Garlic skin (
GAS) has been proven to improve the growth performance of fattening sheep. However, the mechanism by which
GAS affects fattening sheep is not yet clear. The aim of this study is to investigate the effects of adding
GAS to feed on the growth performance, rumen and fecal microbiota, serum and urine metabolism, and transcriptomics of rumen epithelial cells in fattening sheep.
UNASSIGNED: GAS with 80 g/kg dry matter (DM) was added to the diet of fattening sheep to study the effects of GAS on gut microbiota, serum and urine metabolism, and transcriptome of rumen epithelial tissue in fattening sheep. Twelve Hu sheep (body weights; BW, 23.0 ± 2.3 kg and ages 120 ± 3.5 d) were randomly divided into two groups. The CON group was the basal diet, while the
GAS group was supplemented with
GAS in the basal diet. The trial period was 10 weeks, with the first 2 weeks being the pre-trial period.
UNASSIGNED: The daily average weight gain of fattening sheep in the GAS group was significantly higher than that in the CON group (p < 0.05), and the serum GSH-Px of the
GAS group fattening sheep was significantly increased, while MDA was significantly reduced (p < 0.05). Based on the genus classification level, the addition of garlic peel in the diet changed the intestinal microbial composition, and the relative abundance was significantly upregulated by Metanobrevibater (p < 0.05), while significantly downregulated by Akkermansia, Parasutterella, and Guggenheimella (p < 0.05). Metabolomics analysis found that there were 166 significantly different metabolites in serum and 68 significantly different metabolites in urine between the GAS and CON groups (p < 0.05). GAS had an impact on amino acid metabolism, pyrimidine metabolism, methane metabolism, riboflavin metabolism, and unsaturated fatty acid synthesis pathways (p < 0.05). Transcriptome sequencing showed that differentially expressed genes were mainly enriched in immune regulatory function, improving the health of fattening sheep.
UNASSIGNED: Adding GAS can improve the energy metabolism and immune function of fattening sheep by altering gut microbiota, metabolome, and transcriptome, thereby improving the growth performance of fattening sheep.