紫花合欢(舒马赫。)(豆科)是一种用于治疗癫痫和记忆障碍的草药。本研究旨在研究阿比奇水提物对戊四唑(PTZ)诱导的小鼠自发性惊厥的抗惊厥作用;并确定该提取物是否可以减轻记忆障碍。氧化/硝化应激,GABA耗竭和神经炎症。进行超高效液相色谱/质谱分析以鉴定提取物中的活性化合物。每48小时给小鼠注射一次PTZ,直到发生点燃。正常组和阴性对照组动物用蒸馏水,试验组的提取物剂量(40、80或160mg/kg)和阳性对照组的丙戊酸钠剂量(300mg/kg)。记忆是用Y迷宫测量的,新颖的对象识别(NOR)和开放领域范例,而氧化/亚硝化应激(MDA,GSH,CAT,SOD和NO),GABA能传递(GABA,GABA-T和GAD)和神经炎症(TNF-α,IFN-γ,IL-1β,和IL-6)进行测定。还研究了脑显微照片。芹菜素,在提取物中鉴定了murrayanine和savranal。提取物(80-160mg/kg)显著保护小鼠免于PTZ诱导的癫痫发作和死亡。提取物显着提高了Y迷宫和NOR测试中的自发交替和辨别指数,分别。PTZ点燃诱导的氧化/亚硝化应激,GABA耗尽,提取物强烈逆转了神经炎症和神经元细胞死亡。结果表明,合欢花提取物的抗惊厥活性伴随着其抗遗忘特性,并可能得到氧化应激改善的支持,GABA能传递和神经炎症。
Albizia adianthifolia (Schumach.) (Fabaceae) is a medicinal herb used for the treatment of epilepsy and memory impairment. This study aims to investigate the anticonvulsant effects of Albizia adianthifolia aqueous extract against pentylenetetrazole (PTZ)-induced spontaneous convulsions in mice; and determine whether the extract could mitigate memory impairment, oxidative/nitrergic stress, GABA depletion and neuroinflammation. Ultra-high performance liquid chromatography/mass spectrometry analysis was done to identify active compounds from the extract. Mice were injected with PTZ once every 48 h until kindling was developed. Animals received distilled water for the normal group and negative control groups, doses of extract (40, 80, or 160 mg/kg) for the test groups and sodium valproate (300 mg/kg) for the positive control group. Memory was measured using Y maze, novel object recognition (NOR) and open field paradigms, while the oxidative/nitrosative stresses (MDA, GSH, CAT, SOD and NO), GABAergic transmission (GABA, GABA-T and GAD) and neuro-inflammation (TNF-α, IFN-γ, IL- 1β, and IL-6) were determined. Brain photomicrograph was also studied. Apigenin, murrayanine and safranal were identified in the extract. The extract (80-160 mg/kg) significantly protected mice against seizures and mortality induced by PTZ. The extract significantly increased the spontaneous alternation and the discrimination index in the Y maze and NOR tests, respectively. PTZ kindling induced oxidative/nitrosative stress, GABA depletion, neuroinflammation and neuronal cells death was strongly reversed by the extract. The results suggest that the anticonvulsant activity of Albizia adianthifolia extract is accompanied by its anti-amnesic property, and may be supported by the amelioration of oxidative stress, GABAergic transmission and neuroinflammation.