科学证据表明槲皮素(QUR)在实验动物中具有抗焦虑作用。然而,其抗焦虑作用的作用机制尚未发现。这项研究的目的是评估QUR在小鼠模型中的抗焦虑作用,以阐明可能的作用机制。在以50mg/kg(i.p.)的剂量用QUR进行急性腹膜内(i.p.)治疗后,开放领域的行为模型,孔板,吊箱,和明暗测试进行。QUR与GABA能激动剂(地西泮)和/或拮抗剂(氟马西尼)组组合。此外,还进行了计算机模拟分析以观察QUR和GABA(α5)的相互作用,GABA(β1),和GABA(β2)受体。在实验动物模型中,QUR具有抗焦虑作用。QUR,当与地西泮(2mg/kg,i.p.),大大增强了地西泮的抗焦虑作用。QUR是苯二氮卓识别位点的更具竞争性的配体,可以取代氟马西尼(2.5mg/kg,i.p.)。在所有的测试模型中,QUR的作用类似于地西泮,标准抗焦虑药物的作用增强,通过氟马西尼预处理逆转。与GABA(β1)和GABA(β2)受体相比,QUR显示与GABA(α5)受体的最佳相互作用。总之,QUR可能对小鼠产生抗焦虑作用,可能是通过GABA受体相互作用的途径。
Scientific evidence suggests that quercetin (QUR) has anxiolytic-like effects in experimental animals. However, the mechanism of action responsible for its anxiolytic-like effects is yet to be discovered. The goal of this research is to assess QUR\'s anxiolytic effects in mouse models to explicate the possible mechanism of action. After acute intraperitoneal (i.p.) treatment with QUR at a dose of 50 mg/kg (i.p.), behavioral models of open-field, hole board, swing box, and light-dark tests were performed. QUR was combined with a GABAergic agonist (diazepam) and/or antagonist (flumazenil) group. Furthermore, in silico analysis was also conducted to observe the interaction of QUR and GABA (α5), GABA (β1), and GABA (β2) receptors. In the experimental animal model, QUR had an anxiolytic-like effect. QUR, when combined with diazepam (2 mg/kg, i.p.), drastically potentiated an anxiolytic effect of diazepam. QUR is a more highly competitive ligand for the benzodiazepine recognition site that can displace flumazenil (2.5 mg/kg, i.p.). In all the test models, QUR acted similar to diazepam, with enhanced effects of the standard anxiolytic drug, which were reversed by pre-treatment with flumazenil. QUR showed the best interaction with the GABA (α5) receptor compared to the GABA (β1) and GABA (β2) receptors. In conclusion, QUR may exert an anxiolytic-like effect on mice, probably through the GABA-receptor-interacting pathway.