背景:Gα家族在硬骨鱼复杂的生殖调节网络中起着至关重要的作用。然而,Gα家族成员的特征和功能,尤其是Gαq,在硬骨鱼中仍然知之甚少。为了分析特征,表达式,草鱼(Ctenopharyngodonidella)Gαq的功能,我们确定了草鱼基因组中的Gα家族成员,并分析了表达式,分布,和Gαq/gnaq的信号转导。我们还探讨了Gαq在草鱼生殖调节中的作用。
结果:我们的结果表明,草鱼基因组包含27个Gα基因,具有46个亚型,它们分为四个亚科:Gαs,Gαi/o,Gαq/11和Gα12/13。Cignaq在睾丸中的表达程度最高,且显著高于其他组织,其次是下丘脑和大脑。促黄体生成素受体(LHR)主要定位于草鱼卵母细胞的细胞核,信号也存在于卵泡细胞中。相比之下,Gαq信号主要存在于卵母细胞的细胞质中,滤泡细胞中没有信号.在睾丸里,Gαq和LHR共定位在细胞质中。此外,草鱼Gαq重组蛋白显著促进Cipgr的表达。
结论:这些结果为理解Gαq在硬骨鱼生殖调节中的作用提供了初步证据。
BACKGROUND: The Gα family plays a crucial role in the complex reproductive regulatory network of teleosts. However, the characterization and function of Gα family members, especially Gαq, remain poorly understood in teleosts. To analyze the characterization, expression, and function of grass carp (Ctenopharyngodon idella) Gαq, we identified the Gα family members in grass carp genome, and analyzed the expression, distribution, and signal transduction of Gαq/gnaq. We also explored the role of Gαq in the reproductive regulation of grass carp.
RESULTS: Our results showed that the grass carp genome contains 27 Gα genes with 46 isoforms, which are divided into four subfamilies: Gαs, Gαi/o, Gαq/11, and Gα12/13. The expression level of Cignaq in the testis was the highest and significantly higher than in other tissues, followed by the hypothalamus and brain. The luteinizing hormone receptor (LHR) was mainly localized to the nucleus in grass carp oocytes, with signals also present in follicular cells. In contrast, Gαq signal was mainly found in the cytoplasm of oocytes, with no signal in follicular cells. In the testis, Gαq and LHR were co-localized in the cytoplasm. Furthermore, the grass carp Gαq recombinant protein significantly promoted Cipgr expression.
CONCLUSIONS: These results provided preliminary evidence for understanding the role of Gαq in the reproductive regulation of teleosts.