背景:人乳寡糖(HMO),他们的决定因素,婴儿肠道菌群和健康正在进行广泛的研究;然而,很少联合解决。利用HELMi出生队列的数据,我们集体调查了他们,考虑到母婴分泌状态。
方法:使用高效液相色谱法对产后3个月(n=350名母亲)收集的母乳中的HMO成分进行分析。通过鸟枪宏基因组测序,在3、6和12个月(n=823个粪便样本)研究了婴儿肠道微生物群的分类学和功能发育,通过糖苷水解酶(GH)分析重点研究HMO代谢。通过表型和基因分型鉴定母婴分泌状态,分别。儿童健康,强调过敏和抗生素作为传染病的代理,一直记录到2年。
结果:母亲的奇偶校验,肠易激综合征,妊娠期糖尿病,和与HMO成分相关的牛奶收集季节。母婴分泌状态与婴儿肠道菌群无关,除了与个体HMO相关的一些分类群。对出生模式进行分层的分析显示,婴儿肠道菌群和HMO之间存在不同的模式。儿童健康参数与婴儿或母体分泌状态无关。
结论:这项全面的探索揭示了分泌者基因型之间的错综复杂的联系,母性因素,HMO组成,婴儿微生物群,和儿童健康。了解这些细微的关系对于完善优化早期生命营养及其对长期健康的持久影响的策略至关重要。
背景:甜蜜的串扰欧盟H2020MSCAITN,芬兰学院,Mary和GeorgC.Ehrnrooth基金会,Päivikki和SakariSohlberg基金会,还有Tekes.
BACKGROUND: Human milk oligosaccharides (HMOs), their determinants, infant gut microbiota and health are under extensive research; however, seldom jointly addressed. Leveraging data from the HELMi birth cohort, we investigated them collectively, considering maternal and infant secretor status.
METHODS: HMO composition in breastmilk collected 3 months postpartum (n = 350 mothers) was profiled using high-performance liquid chromatography. Infant gut microbiota taxonomic and functional development was studied at 3, 6, and 12 months (n = 823 stool samples) via shotgun metagenomic sequencing, focusing on HMO metabolism via glycoside hydrolase (GH) analysis. Maternal and infant secretor statuses were identified through phenotyping and genotyping, respectively. Child health, emphasizing allergies and antibiotics as proxies for infectious diseases, was recorded until 2 years.
RESULTS: Mother\'s parity, irritable bowel syndrome, gestational diabetes, and season of milk collection associated with HMO composition. Neither maternal nor infant secretor status associated with infant gut microbiota, except for a few taxa linked to individual HMOs. Analysis stratified for birth mode revealed distinct patterns between the infant gut microbiota and HMOs. Child health parameters were not associated to infant or maternal secretor status.
CONCLUSIONS: This comprehensive exploration unveils intricate links between secretor genotype, maternal factors, HMO composition, infant microbiota, and child health. Understanding these nuanced relationships is paramount for refining strategies to optimize early life nutrition and its enduring impact on long-term health.
BACKGROUND: Sweet Crosstalk EU H2020 MSCA ITN, Academy of Finland, Mary and Georg C. Ehrnrooth Foundation, Päivikki and Sakari Sohlberg Foundation, and Tekes.