由TilletialaevisKühn引起的普通unt是小麦最严重的真菌病害之一。根微生物协会在保护植物免受生物和非生物因素的影响中起着关键作用。管理这些协会为提高农业生产的可持续性和效率提供了平台。这里,通过使用高通量测序,我们旨在鉴定小麦中的细菌和真菌关联,苜蓿,和中国甘肃省不同年份种植的燕麦作物。根据Chao1分析,来自T.laevis感染的小麦的土壤样品(表面以下0-6cm)的细菌和真菌丰富度明显高于对照样品。我们在T.laevis感染的小麦中发现了一些优势真菌和细菌门,比如变形杆菌,酸杆菌,放线菌,氯氟菌,子囊,担子菌,还有Mortierllomycota.我们还分析了T.laevis接种后土壤样品的化学和酶学性质。总氮,总钾(TK),铵态氮,可用的钾,有机碳,转化酶,磷酸酶与对照样品相比,感染了T.laevis的样品中过氧化氢酶更多,而pH值,总磷,硝酸盐氮,有效磷,与感染T.laevis的样品相比,对照样品中的脲酶更多。这项研究的结果将有助于通过候选拮抗微生物和土壤的不利特性来控制小麦的普通unt。
Common bunt caused by Tilletia laevis Kühn is one of the most serious fungal diseases of wheat. The root-microbial associations play key roles in protecting plants against biotic and abiotic factors. Managing these associations offers a platform for improving the sustainability and efficiency of agriculture production. Here, by using high throughput sequencing, we aimed to identify the bacterial and fungal associations in wheat, alfalfa, and oat crops cultivated in different years in the Gansu province of China. Soil samples (0-6 cm below the surface) from infected wheat by T. laevis had significantly more bacterial and fungal richness than control samples as per the Chao1 analysis. We found some dominant fungi and bacterial phyla in infected wheat by T. laevis, such as Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Ascomycota, Basidiomycota, and Mortierello mycota. We also analyzed the chemical and enzymatic properties of soil samples after T. laevis inoculation. The total nitrogen, total kalium (TK), ammonium nitrogen, available kalium, organic carbon, invertase, phosphatase, and catalase were more in T. laevis-infected samples as compared to the control samples, while pH, total phosphorus, nitrate nitrogen, available phosphorus, and urease were more in control samples compared to T. laevis-infected samples. The results of this study will contribute to the control of wheat common bunt by candidate antagonistic microorganisms and adverse properties of soil.