UNASSIGNED:为了评估用羧甲基纤维素(CMC)包埋和用最佳切割温度(OCT)化合物包埋,然后用PBS(OCT-W)洗涤的潜在作用,用解吸电喷雾电离质谱成像(DESI-MSI)分析乳腺癌组织样品。
未经证实:新鲜冷冻(FF)组织样本的DESI-MSI,OCT嵌入样本,CMC嵌入样品,和来自相同乳腺癌肿瘤组织的OCT-W样品。最大丰度离子的比率用于评估DESI-MSI分析的再现性。此外,通过比较特征离子种类和DESI-MSI检测到的离子信号强度来检查每组的治疗效果。
UNASSIGNED:FF样品连续切片的DESI-MSI表明,m/z281.25,m/z309.28和m/z279.23离子的对对比的变异系数(CV),肿瘤区域中强度最高的三种离子,是19.61%,20.74%和10.18%,分别。CMC包埋组织和OCT-W组织的DESI-MSI检测到的特征离子种类几乎相同,与FF肿瘤组织相比。然而,在OCT嵌入样品中检测到的离子种类少于FF样品的50%。就离子信号强度而言,CMC包埋组织总体上没有受到影响,而OCT-W组的大部分特征离子的信号强度降低(P<0.05)。
UNASSIGNED:FF组织切片和CMC包埋的样品可用于DESI-MSI常规分析。无论样品是否经过PBS洗涤,OCT包埋都会影响样品分析的可行性。如果由于样本量小,组织切片需要固定和支撑,建议使用CMC包埋剂。脆弱,或其他问题。
UNASSIGNED: To evaluate the potential effect of embedding with carboxylmethyl cellulose (CMC) and embedding with optimal cutting temperature (OCT) compound followed by washing with PBS (OCT-W) on the analysis of breast cancer tissue samples with desorption electrospray ionization mass spectrometry imaging (DESI-MSI).
UNASSIGNED: DESI-MSI of fresh frozen (FF) tissue samples, OCT-embedded samples, CMC-embedded samples, and OCT-W samples from the same breast cancer tumor tissue were performed. The ratio of maximum abundance ion was used to assess the reproducibility of DESI-MSI analysis. In addition, the effects of the treatment of each group were examined by comparing the characteristic ion species and the ion signal intensity detected by DESI-MSI.
UNASSIGNED: DESI-MSI of continuous sections of FF samples showed that the coefficient of variation (CV) of the pair-to-pair ratios of m/ z 281.25, m/ z 309.28 and m/ z 279.23 ions, the three ions with the highest intensity in the tumor region, were 19.61%, 20.74% and 10.18%, respectively. The characteristic ion species detected by DESI-MSI of CMC embedded tissue and the OCT-W tissue were almost the same, compared with those of the FF tumor tissue. However, ion species detected in OCT embedded samples were less than 50% of the FF samples. In terms of ion signal intensity, the CMC embedded tissue was not affected overall, while the signal of most of the characteristic ions of the OCT-W group showed decreased intensity (P<0.05).
UNASSIGNED: FF tissue sections and CMC-embedded samples can be used for DESI-MSI routine analysis. OCT embedding affects the feasibility of sample analysis whether or not the sample undergoes washing with PBS. CMC embedding agent is recommended if the tissue sections need to be fixated and supported due to small sample size, fragility, or other problems.