背景:甘氨酸maxL.与Drakshasava,在印度卡纳塔克邦北部农村地区,传统治疗师广泛用作治疗消化性溃疡的制剂,根据患者评估和我们先前发表的传统使用制剂的研究工作,似乎是有效的。
目的:进行本研究以评估过夜水浸泡的G.max谷物的安全性和有效性。这是传统配方的组成部分之一。这项研究,在评估其对小鼠的毒性后,在雄性Wistar大鼠中进行了机构动物伦理委员会的批准。
方法:4组大鼠(每组n=6)口服阿司匹林200mg/kg。对照组除服用阿司匹林外,还服用生理盐水,标准组接受20mg/kg奥美拉唑,第3(第)和第4(第)组接受G.max250和500mg/kg,分别。所有治疗每24小时口服给药7天。禁食24小时后,在第8(第)天,在麻醉下抽吸胃内容物,以估计游离和总酸度。沿着较大的曲率打开胃,以计算溃疡指数并进行组织病理学研究。
方法:通过单因素方差分析和Dunnett的事后检验对结果进行分析。P≤0.05被认为是显著的。
结果:实验组与对照组相比,阿司匹林引起的溃疡严重程度明显降低(P<0.05)。500mg/kg治疗组的游离酸度和总酸度显着降低,与对照组相比,疗效优于奥美拉唑治疗组。
结论:发现G.max颗粒对阿司匹林引起的溃疡有效。
BACKGROUND: Glycine max L. with Drakshasava, widely used by traditional healers as a formulation for the treatment of peptic ulcer in rural northern Karnataka in India, appears to be effective as assessed by patients and in our previously published research work of traditionally used formulation.
OBJECTIVE: The present study was undertaken to evaluate the safety and efficacy of the overnight water soaked G. max grains. This is one of the components of traditional formulation. The study, approved by Institutional Animal Ethics Committee was carried out in male Wistar rats after assessing its toxicity in mice.
METHODS: Four groups of rats (n = 6 in each group) were treated with aspirin 200 mg/kg oral. In addition to aspirin control group received normal saline, standard group received 20 mg/kg omeprazole and 3(rd) and 4(th) group received G. max 250 and 500 mg/kg, respectively. All treatments were administered orally every 24 h for 7 days. After 24 hours fasting, on the 8(th) day stomach contents were aspirated under anesthesia to estimate free and total acidity. Stomachs were opened along the greater curvature to calculate ulcer index and subjected to histopathology studies.
METHODS: The results were analyzed by one-way analysis of variance followed-by Dunnett\'s post hoc test. P ≤0.05 was considered as significant.
RESULTS: The severity of aspirin induced ulceration was found significantly (P < 0.05) decreased in test groups compared with the control group. Free and total acidity was significantly reduced in 500 mg/kg treated group, compared with the control group and was inferior to omeprazole treated group.
CONCLUSIONS: The grain of G. max was found to be effective against aspirin induced ulcers.