台湾鱼肉是一种刺激性的吸血吸虫,优先攻击人类并影响台湾的休闲产业。了解此类害虫的雌性生殖生物学将有助于制定害虫控制策略。然而,尚不了解有关产卵菌属的卵子发生的知识。因此,我们从卵母细胞和护士细胞的角度检查了卵巢的结构和卵子发生的特征。血液喂养后,我们观察到高度的促性腺激素和谐-发育中卵泡的同步性。F.taiwana的卵泡只有一个护士细胞与卵母细胞相连,这在吸血mid中截然不同。在护士牢房里,我们确定了种系标记的核周定位,瓦萨.Vasa定位让人想起通过电子显微镜观察到的与核包膜相关的nuage。为了确定F.taiwanaVasa(FtVasa)是否是真实的营养成分,我们生产了在雌性种系中表达FtVasa的转基因果蝇,并证明了FtVasa能够定位于果蝇nuage。通过表征F.taiwana种系细胞中的卵子发生和Vasa表达,这项研究扩展了有关吸血mid的女性生殖生物学的知识。
Forcipomyia taiwana is an irritating hematophagous midge that preferentially attacks humans and affects leisure industries in Taiwan. Understanding the female reproductive biology of such pests would facilitate the development of pest control strategies. However, knowledge about oogenesis in the genus
Forcipomyia is unavailable. Accordingly, we examined the ovariole structure and features of oogenesis in terms of the oocyte and the nurse cell. After being blood-fed, we observed a high degree of gonotrophic harmony-the synchronization of developing follicles. The follicle of the F. taiwana has only one nurse cell connected to the oocyte, which is distinct among hematophagous midges. In the nurse cell, we identified the perinuclear localization of the germline marker, Vasa. The Vasa localization is reminiscent of the nuclear envelope-associated nuage observed by electron microscopy. To determine whether F. taiwana Vasa (FtVasa) is an authentic nuage component, we produced transgenic flies expressing FtVasa in the female germline and proved that FtVasa was able to be localized to Drosophila nuage. By characterizing the oogenesis and Vasa expression in the germline cells of F. taiwana, this study extends knowledge about the female reproductive biology of hematophagous midges.