SO2衍生物,亚硫酸盐/亚硫酸氢盐,广泛应用于食品加工和药物合成行业。尽管它们广泛应用,过量的亚硫酸盐/亚硫酸氢盐会对人体健康产生负面影响。大多数用于检测亚硫酸盐/亚硫酸氢盐的探针由于其在可见光谱范围内的荧光和在水溶液中的溶解性差而受到限制,这限制了它们在食品测试和生物成像中的使用。在这里,由氰基吡啶菁骨架组成的近红外探针,4-((Z)-2-((E)-2-氯-3-(2-氰基-2-(1-甲基吡啶-4(1H)-亚基)亚乙基)环己-1-烯-1-基)-1-氰基乙烯基)-1-甲基吡啶-1-铵(简写为CCP),已开发。该探针能够精确定量几乎纯缓冲溶液中的亚硫酸氢盐(HSO3-),在784nm处显示近红外荧光发射,检测限低至0.32μM。该探针因其出色的选择性而脱颖而出,对干扰的敏感性最小,适应性强。探针CCP利用CC键通过亲核加成引发与HSO3-的近红外荧光猝灭反应,这有效地破坏了分子内的大量离域,以进行准确的HSO3-鉴定。此外,该探针已成功应用于检测各种食品和活细胞中的HSO3-,简化了水样中HSO3-含量的测量。这种进步不仅增强了分析能力,而且有助于确保食品安全和环境保护。环境含义:SO2衍生物,包括亚硫酸盐/亚硫酸氢盐,兼有防腐剂和抗氧化剂的双重作用,广泛应用于各个领域,包括食品防腐,水卫生,和制药业。尽管它们广泛应用,过量的亚硫酸盐/亚硫酸氢盐会影响人体健康。开发精确和灵敏地检测食品和生物样品中亚硫酸盐/亚硫酸氢盐的方法对于确保食品安全和环境保护非常重要。这里,在99.9%缓冲溶液中灵敏的近红外和多功能荧光探针,随着水凝胶封装,已成功应用于食品中亚硫酸氢盐的检测,正宗水样,和生物细胞。
SO2 derivatives, sulfite/bisulfite, are widely employed in both the food processing and drug synthesis industries. Despite their widespread application, excessive levels of sulfite/bisulfite can negatively impact human health. Most probes for detecting sulfite/bisulfite are restricted by their fluorescence within the visible spectrum range and poor solubility in aqueous solution, which limit their use in food testing and biological imaging. Herein, a near-infrared probe comprising of the cyanopyridine cyanine skeleton, 4-((Z)-2-((E)-2-chloro-3-(2-cyano-2-(1-methylpyridine-4(1H)-ylidene)ethylidene)cyclohex-1-en-1-yl)-1-cyanovinyl)-1-methylpyridin-1-ium (abbreviated as CCP), was developed. This probe enables precise quantification of bisulfite (HSO3-) in almost pure buffered solutions, showing a near-infrared fluorescence emission at 784 nm with an impressively low detection limit of 0.32 μM. The probe stands out for its exceptional selectivity, minimal susceptibility to interference, and strong adaptability. The probe CCP utilizes the CC bond to trigger a near-infrared fluorescence quenching reaction with HSO3- via nucleophilic addition, which effectively disrupts the large delocalization within the molecule for accurate HSO3- identification. Moreover, the probe has been successfully applied in detecting HSO3- in various food products and living cells, simplifying the measurement of HSO3- content in water samples. This advancement not only enhances the analytical capabilities but also contributes to ensuring food safety and environmental protection. ENVIRONMENTAL IMPLICATION: SO2 derivatives including sulfite/bisulfite, serving dual roles as preservatives and antioxidants, have widespread application across various sectors including food preservation, water sanitation, and the pharmaceutical industry. Despite their widespread application, excessive levels of sulfite/bisulfite can affect human health. Developing methods for precisely and sensitively detecting sulfite/bisulfite in food products and biological samples is important for ensuring food safety and environmental protection. Here, a sensitive near-infrared and multifunctional fluorescent probe in a 99.9 % buffered solution, along with water gel encapsulation, has been successfully applied for the detection of bisulfite in food, authentic water samples, and biological cells.