信息素结合蛋白(PBP)是可以特异性识别昆虫信息素的特异性气味结合蛋白。通过对成年Endoclita信号触角的转录分析,发现并鉴定了EsigPBP3,发现EsigPBP3在雄蛾的触角中高度表达。根据EsigPBP3的结合特性和能力,我们可以找到关键配体和结合位点来考虑作为控制关键木孔E.signifier的靶标。在这项研究中,荧光竞争结合试验(FCBA)表明,EsigPBP3对7种关键桉树挥发物有很高的结合亲和力。分子对接分析表明,EsigPBP3对性信息素成分具有最强的结合亲和力,(3E,7E)-4,7,11-三甲基-1,3,7,10-十二碳烯。此外,与FCBA的结果相同,EsigPBP3对关键桉树挥发物表现出高结合亲和力,桉树脑,α-萜品烯,(E)-β-新烯,(-)-β-品烯,和(-)-α-pine烯,PHE35、MET7、VAL10、PHE38、ILE52和PHE118是关键位点。总之,EsigPBP3对雄性信息素和关键挥发性化合物具有高结合亲和力,关键结合位点PHE35,MET7,VAL10,PHE38,ILE52和PHE118可以作为识别E.signifier信息素的靶标。
Pheromone-binding proteins (PBPs) are specific odorant-binding proteins that can specifically recognize insect pheromones. Through transcriptional analysis of the antennae of adult Endoclita signifer, EsigPBP3 was discovered and identified, and EsigPBP3 was found to be highly expressed in the antennae of male moths. Based on the binding characteristics and ability of EsigPBP3, we can find the key ligands and binding site to consider as a target to control the key wood bore E. signifier. In this study, the fluorescence competitive binding assays (FCBA) showed that EsigPBP3 had a high binding affinity for seven key eucalyptus volatiles. Molecular docking analysis revealed that EsigPBP3 had the strongest binding affinity for the sexual pheromone component, (3E,7E)-4,7,11-trimethyl-1,3,7,10-dodecatetraene. Furthermore, same as the result of FCBA, the EsigPBP3 exhibited high binding affinities to key eucalyptus volatiles, eucalyptol, α-terpinene, (E)-beta-ocimene, (-)-β-pinene, and (-)-α-pinene, and PHE35, MET7, VAL10, PHE38, ILE52, and PHE118 are key sites. In summary, EsigPBP3 exhibits high binding affinity to male pheromones and key volatile compounds and the crucial binding sites PHE35, MET7, VAL10, PHE38, ILE52, and PHE118 can act as targets in the recognition of E. signifier pheromones.