胎盘血管生成是胎儿-母体循环的关键过程,并确保整个妊娠期间胎盘的有效发育。体外受精和胚胎移植过程中的许多因素可能会影响胎盘基因表达和胎儿发育。本研究旨在鉴定血管生成相关基因(VEGFA,健康女性辅助生殖技术受精和自然受孕后胎盘中FGF2,FLT1和KDR)的表达谱。在一项病例对照研究中,从辅助生殖技术受精(N=20)和无并发症妊娠妇女自然受孕(N=9)后的高加索妇女中收集足月胎盘。检测胎盘中VEGFA的mRNA表达,FGF2,FLT1和KDR基因通过实时定量聚合酶链反应(RT-qPCR)。进行组分层,以比较转移的胚胎类型(新鲜/冷冻)之间的研究基因。组织捐献的地点(中心/边缘),和新生儿\'性别(男性/女性)。在ART胎盘中,观察到VEGFA基因的显著下调(p=0.016)和FLT1(p=0.026)和KDR(p<0.001)基因受体的上调。与对照相比,编码VEGFA受体的基因在新鲜(ET)和冷冻(FET)胚胎移植组中都上调。对于FLT1基因,在冷冻胚胎移植组和对照组之间观察到统计学上的显著差异(p=0.032).与对照组相比(p<0.001)和ET和FET之间(p=0.002),两个胚胎移植组的KDR相对表达显著更高。在不同的组织捐献地点和新生儿性别中,胎盘表达无统计学差异。我们观察到与自然受孕相比,辅助生殖技术后妊娠中VEGFA及其受体FLT1和KDR的胎盘表达差异。需要更多的研究来阐明这些可能影响胎盘发育和胎儿健康的改变。
Placental angiogenesis is a pivotal process for feto-maternal circulation and ensures efficient development of the placenta throughout pregnancy. Many factors during in vitro fertilization and embryo transfer procedures may affect placental gene expression and fetus development. The present study aimed to identify differences in angiogenesis-related gene (VEGFA, FGF2,
FLT1, and KDR) expression profiles in placentas after assisted reproductive technology fertilization and natural conception in healthy women. In a case-control study, term placentas were collected from Caucasian women after assisted reproductive technology fertilization (N = 20) and after natural conception in women with uncomplicated pregnancy (N = 9). The mRNA expression in placentas was examined for VEGFA, FGF2,
FLT1, and KDR genes by real-time quantitative polymerase chain reaction (RT-qPCR). Group stratification was performed for comparison of investigated genes between the type of embryo transferred (fresh/frozen), place of tissue donation (center/margin), and newborns\' gender (male/female). In the ART placentas, significant down-regulation of VEGFA gene (p = 0.016) and up-regulation of
FLT1 (p = 0.026) and KDR (p < 0.001) gene receptors were observed. Genes encoding VEGFA receptors were up-regulated in both fresh (ET) and frozen (FET) embryo transfer groups compared to controls. For the
FLT1 gene, a statistically significant difference was observed between the frozen embryo transfer group and the controls (p = 0.032). Relative expression of KDR was significantly higher for both embryo transfer groups compared to controls (p < 0.001) and between ET and FET (p = 0.002). No statistically significant differences were observed between placental expression in different places of tissue donation and newborns\' gender. We observed differences in the placental expression of VEGFA and its receptors
FLT1 and KDR in pregnancies after assisted reproductive technology compared to naturally conceived pregnancies. More research is needed to clarify these alterations that may affect placental development and fetal health.