在最近的过去,各种微藻被认为是生物燃料生产的可再生能源,它们的数量和程度可以通过施加某些类型的胁迫来增强,包括盐度。尽管在盐度胁迫下生长的微藻会导致较高的脂质含量,它们同时减少了生长和生物量输出。要解决此问题,微藻Scenedesmussp.的生理化学变化。BHU1已通过两阶段培养进行了评估。在第一阶段,在0.4MNaCl浓度下发现最大的碳水化合物和脂质含量(39.55和34.10%),在第二阶段,在8天大的培养物中获得了最大的碳水化合物和脂质含量(42.16和38.10%)。然而,在盐度增加的情况下,Scenedesmussp.BHU1表现出光合属性的降低,包括Chl-a,Chl-b,Fv/Fm,Y(II),Y(NPQ),NPQ,qP,qL,qN,和ETRmax,但增加Y(NO)和类胡萝卜素含量。除了生理属性,渗透保护剂,压力生物标志物,还研究了非酶抗氧化剂,以阐明活性氧(ROS)促进脂质合成的作用。此外,进行了微藻生物质的元素和矿物离子分析,以评估生物燃料和细胞稳态的生物质质量。基于荧光分析,在Scenedesmussp.的第二阶段,我们在8天大的培养物中发现了最大的中性脂质。BHU1.此外,傅立叶变换红外(FT-IR)和核磁共振(NMR)光谱分析的使用证实了由饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)组成的较高水平的碳氢化合物和三酰基甘油酯(TAG)在8天大的培养物中。因此,Scenedesmussp.BHU1可以是潜在的生物柴油原料的有前途的微藻。
In the recent past, various microalgae have been considered a renewable energy source for biofuel production, and their amount and extent can be enhanced by applying certain types of stress including salinity. Although microalgae growing under salinity stress result in a higher lipid content, they simultaneously reduce in growth and biomass output. To resolve this issue, the physiochemical changes in microalgae Scenedesmus sp. BHU1 have been assessed through two-stage cultivation. In stage-I, the maximum carbohydrate and lipid contents (39.55 and 34.10%) were found at a 0.4 M NaCl concentration, while in stage-II, the maximum carbohydrate and lipid contents (42.16 and 38.10%) were obtained in the 8-day-old culture. However, under increased salinity, Scenedesmus sp. BHU1 exhibited a decrease in photosynthetic attributes, including Chl-a, Chl-b, Fv/Fm, Y(II), Y(NPQ), NPQ, qP, qL, qN, and ETRmax but increased Y(NO) and carotenoids content. Apart from physiological attributes, osmoprotectants, stress biomarkers, and nonenzymatic antioxidants were also studied to elucidate the role of reactive oxygen species (ROS) facilitated lipid synthesis. Furthermore, elemental and mineral ion analysis of microalgal biomass was performed to evaluate the biomass quality for biofuel and cell homeostasis. Based on fluorometry analysis, we found the maximum neutral lipids in the 8-day-old grown culture at stage-II in Scenedesmus sp. BHU1. Furthermore, the use of Fourier-transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopy analyses confirmed the presence of higher levels of hydrocarbons and triacylglycerides (TAGs) composed of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in the 8-day-old culture. Therefore, Scenedesmus sp. BHU1 can be a promising microalga for potential biodiesel feedstock.