■p16在细胞核和细胞质位置中具有不同的作用。p16蛋白的核定位解释了其在细胞周期调控中的作用。细胞质表达在最初几年被认为是一种伪影,但是有证据证明细胞质定位是真实的,并且p16在细胞核和细胞质位置具有不同的作用。我们旨在研究p16蛋白在纤维腺瘤上皮和基质区室的细胞核和细胞质位置的免疫表达,浸润性乳腺癌,和一定数量的叶状肿瘤。
■该研究共包括107名患者,包括51例浸润性乳腺癌,纤维腺瘤51例,良性叶状肿瘤4例,小叶原位癌(LCIS)1例。评估了p16免疫组织化学在肿瘤上皮和基质区室中的核和细胞质定位。
■在51例纤维腺瘤中,23显示出强的核p16上皮表达,但无病例显示细胞质表达。在19/51案例中,基质细胞也显示出强的p16核表达。在4例良性叶状中的3例中观察到中度基质p16表达。在51例浸润性癌中,31显示中强核p16免疫阳性,27例显示细胞质p16表达。我们发现中度至强细胞核p16免疫表达与乳腺癌分子亚型之间存在统计学上的显着相关性。
■在纤维腺瘤的上皮成分中未发现p16免疫组织化学的细胞质定位,而它常见于乳腺癌。核p16表达与乳腺癌的分子亚型有统计学意义。
UNASSIGNED: p16 has different roles in the nuclear and cytoplasmic locations. The nuclear localization of the p16 protein explains its role in cell cycle regulation. Cytoplasmic expression was considered an artifact in the initial years, but there is evidence to prove that cytoplasmic localization is real and that p16 has different roles in the nuclear and cytoplasmic locations. We aimed to study the immunoexpression of p16 protein in the nuclear and cytoplasmic locations of the epithelial and stromal compartments of
fibroadenoma, invasive breast carcinoma, and a select number of phyllodes tumors.
UNASSIGNED: The study included a total of 107 patients, comprising 51 cases of invasive breast carcinoma, 51 cases of
fibroadenoma, 4 cases of benign phyllodes tumors, and 1 case of lobular carcinoma in situ (LCIS). The p16 immunohistochemistry was evaluated for nuclear and cytoplasmic localization in the epithelial and stromal compartments of the tumors.
UNASSIGNED: Of the 51
fibroadenoma cases, 23 showed strong nuclear p16 epithelial expression, but no case showed cytoplasmic expression. In 19/51 cases, stromal cells also showed strong p16 nuclear expression. Moderate stromal p16 expression was observed in 3 out of 4 cases of benign phyllodes. Out of the 51 cases of invasive carcinoma, 31 showed moderate to strong nuclear p16 immunopositivity, while 27 cases exhibited cytoplasmic p16 expression. We found a statistically significant correlation between moderate to strong nuclear p16 immunoexpression and the molecular subtype of breast carcinoma.
UNASSIGNED: The cytoplasmic localization of p16 immunohistochemistry is not seen in epithelial components of
fibroadenoma, while it is seen frequently in breast carcinoma. Nuclear p16 expression has a statistically significant correlation with molecular subtypes of breast carcinoma.