目标-金属元素和纤维蛋白凝块特性与中风有关。我们检查了金属和非金属元素,纤维蛋白凝块溶解时间(CLT),和最大吸光度(Absmax)与缺血性卒中的关系。设计-缺血性卒中患者与患者的病例对照研究健康的个体。受试者和方法-收集260例缺血性卒中患者的血浆和血清(45.0%为女性;年龄,68±12岁)和291名健康对照(59.7%的女性;年龄,50±17岁)。使用经验证的比浊法测定纤维蛋白CLT和Absmax。通过电感耦合等离子体质谱法(ICP-MS)和发射光谱法(ICP-OES)对血清元素进行定量。通过双变量相关性和多元回归或逻辑回归分析数据。结果-在女性中风患者中,铜,锂,与对照组相比,铝明显较低;在男性中风患者中,钾较低,铍升高了.在女性和男性中风患者中,铁,锌,镍,钙,镁,钠,硅含量明显较低,而锶升高。纤维蛋白凝块特性与金属呈正相关,在健康对照中观察到,在缺血性中风患者中丢失。在多元回归分析中,纤维蛋白CLT和/或Absmax与锌有关,钙,钾,铍,中风患者的硅和钠,钾,铍,和控制中的铝。在逻辑回归分析中,卒中与锂独立相关,镍,铍,锶,硼,硅和钠,钾,钙,和铝,但不含纤维蛋白CLT/Absmax。结论:多种因素与纤维蛋白凝块特性和缺血性卒中风险相关。锂,钠,钙,和铝消除了纤维蛋白凝块特性与缺血性卒中的关联。
Objectives-Metallic elements and fibrin clot properties have been linked to stroke. We examined metallic and nonmetallic elements, fibrin clot lysis time (CLT), and maximum absorbance (Absmax) in relation to ischemic stroke. Design-A case-control study of ischemic stroke patients vs. healthy individuals. Subjects and Methods-Plasma and serum were collected from 260 ischemic stroke patients (45.0% women; age, 68 ± 12 years) and 291 healthy controls (59.7% women; age, 50 ± 17 years). Fibrin CLT and Absmax were measured using a validated turbidimetric assay. Serum elements were quantified by inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES). Data were analyzed by bivariate correlations and multiple or logistic regression. Results-In female stroke patients, copper, lithium, and aluminum were significantly lower compared with controls; in male stroke patients, potassium was lower, and beryllium was elevated. In female and male stroke patients, iron, zinc, nickel, calcium, magnesium, sodium, and silicon were significantly lower, while strontium was elevated. Positive correlations between fibrin clot properties and metals, observed in healthy controls, were lost in ischemic stroke patients. In multivariate regression analysis, fibrin CLT and/or Absmax was associated with zinc, calcium, potassium, beryllium, and silicon in stroke patients and with sodium, potassium, beryllium, and aluminum in controls. In logistic regression analysis, stroke was independently associated with lithium, nickel, beryllium, strontium, boron, and silicon and with sodium, potassium, calcium, and aluminum but not with fibrin CLT/Absmax. Conclusions-Various elements were associated with fibrin clot properties and the risk of ischemic stroke. Lithium, sodium, calcium, and aluminum abrogated the association of fibrin clot properties with ischemic stroke.