近年来,美国养猪业的母猪死亡率有所增加,盆腔器官脱垂(POP)是主要原因,占所有母猪死亡率的21%。微生物群落的菌群失调与几种物种的疾病和生殖功能障碍有关,和以前的研究表明,在妊娠后期发生POP风险增加的母猪阴道微生物群的变化。然而,关于母猪粪便微生物群与POP之间潜在关系的知识不足。因此,本研究的目的是确定母猪粪便微生物群的差异,并确定粪便和阴道微生物群落是否与POP风险相关.
■使用已建立的会阴评分系统评估母猪的POP风险,会阴评分(PS)为1(PS1)假定POP的风险很小或没有,而PS为3(PS3)则假定POP的风险很高。在目前的研究中,2,864头母猪在妊娠第15周得分,为1.0%,2.7%,和23.4%的PS1、PS2和PS3母猪,分别,后来经历了POP。在妊娠第108-115天之间收集粪便拭子(n=215),提取DNA,使用mothur分析16SrRNA基因扩增子测序文库,phyloseq和SAS参考PS和POP结果。此外,使用CoNet构建共现网络,以比较来自相同母猪队列的粪便和阴道微生物群,并确定不同分类群之间的相关性.
■粪便群落组成差异(PERMANOVA;P<0.05),结构(α多样性测量;P<0.05),在PS1和PS3分配的母猪之间显示了13个单独的操作分类单位(OTU)。由于POP结果,未检测到粪便微生物群的差异。然而,几个类群的丰度在样本采集地点之间是相关的,这表明粪便和阴道微生物群落可能彼此相关。
■集体,与阴道微生物群相比,POP风险不同的母猪的粪便微生物群差异较少,提示阴道微生物组可能与POP结果更相关,尽管粪便和阴道群落之间的相关性可以为抗击POP的策略提供见解。
UNASSIGNED: Sow mortality in the U.S. swine industry has increased in recent years, for which pelvic organ prolapse (POP) is a major contributor, accounting for 21% of all sow mortality. Dysbiosis of microbial communities has been associated with disease and reproductive dysfunction in several species, and previous studies have shown changes in vaginal microbiota in sows with increased risk for POP during late gestation. However, there is insufficient knowledge surrounding the potential relationship between fecal microbiota and POP in sows. Therefore, the study objective was to identify differences in sow fecal microbiota and determine if fecal and vaginal microbial communities are correlated in relation to POP risk.
UNASSIGNED: Sows were evaluated for POP risk using an established perineal scoring system, with a perineal score (PS) of 1 (PS1) presuming little to no risk of POP to a PS of 3 (PS3) presuming high risk of POP. In the current study, 2,864 sows were scored during gestation week 15, and 1.0%, 2.7%, and 23.4% of PS1, PS2, and PS3 sows, respectively, subsequently experienced POP. Fecal swabs (n = 215) were collected between gestation days 108-115, DNA was extracted, and 16S rRNA gene amplicon sequencing libraries were analyzed using mothur, phyloseq and SAS in reference to PS and POP outcome. Additionally, co-occurrence networks were constructed using CoNet to compare fecal and vaginal microbiota from the same cohort of sows and identify correlations between different taxa.
UNASSIGNED: Differences in fecal community composition (PERMANOVA; P < 0.05), structure (alpha diversity measurements; P < 0.05), and 13 individual operational taxonomic units (OTUs) were revealed between PS1 and PS3 assigned sows. No differences in fecal microbiota were detected as a result of POP outcome. However, the abundances of several taxa were correlated across sample collection sites, suggesting the fecal and vaginal microbial communities may be related to one another.
UNASSIGNED: Collectively, fewer differences in the fecal microbiota exist in sows with differing risk for POP compared to the vaginal microbiota, suggesting the vaginal microbiome may be more relevant in relation to POP outcome, although correlations between fecal and vaginal communities may provide insight for strategies to combat POP.