目的:我们假设同时掺入离子通道肽(在这种情况下,钾通道作为模型)和疏水性磁铁矿Fe3O4纳米颗粒(hFe3O4NPs)在脂质六角形中间相内,使用外部磁场排列它们可以显着增强离子通过脂质膜的转运。
方法:在本研究中,我们使用SAXS和cryo-TEM方法成功地表征了在脂质六角形结构中掺入的小草素膜离子通道和hFe3O4NP。此外,我们彻底研究了独立膜的脂质六方中间相的导电特性,有和没有小草菌素钾通道,利用一系列电化学技术,包括阻抗谱,正常脉冲伏安法,和计时电流法.
结果:我们的研究揭示了在作为整合蛋白质和肽的基质的溶致液晶中增强离子转运的最新突破。我们证明了由嵌有K转运肽的六角形脂质中间相组成的膜的显着功效。这种增强通过用hFe3O4NP掺杂和暴露于磁场来实现。我们研究了脂质六边形结构的导电特性之间的复杂相互作用,hFe3O4NP,短句来源以及Ca2+对K+通道的影响。此外,我们的研究揭示了离子通道研究和仿生膜研究的新方向,为仿生膜提供了一种通用模型,在适当定向的磁场下具有前所未有的离子传输能力。这些发现有望促进膜技术以及膜蛋白的各种生物技术和生物医学应用。
OBJECTIVE: We hypothesize that simultaneous incorporation of ion channel peptides (in this case, potassium channel as a model) and hydrophobic magnetite Fe3O4 nanoparticles (hFe3O4NPs) within lipidic hexagonal mesophases, and aligning them using an external magnetic field can significantly enhance ion transport through lipid membranes.
METHODS: In this study, we successfully characterized the incorporation of gramicidin membrane ion channels and hFe3O4NPs in the lipidic hexagonal structure using SAXS and cryo-TEM methods. Additionally, we thoroughly investigated the conductive characteristics of freestanding films of lipidic hexagonal mesophases, both with and without gramicidin potassium channels, utilizing a range of electrochemical techniques, including impedance spectroscopy, normal pulse voltammetry, and chronoamperometry.
RESULTS: Our research reveals a state-of-the-art breakthrough in enhancing ion transport in lyotropic liquid crystals as matrices for integral proteins and peptides. We demonstrate the remarkable efficacy of membranes composed of hexagonal lipid mesophases embedded with K+ transporting peptides. This enhancement is achieved through doping with hFe3O4NPs and exposure to a magnetic field. We investigate the intricate interplay between the conductive properties of the lipidic hexagonal structure, hFe3O4NPs, gramicidin incorporation, and the influence of Ca2+ on K+ channels. Furthermore, our study unveils a new direction in ion channel studies and biomimetic membrane investigations, presenting a versatile model for biomimetic membranes with unprecedented ion transport capabilities under an appropriately oriented magnetic field. These findings hold promise for advancing membrane technology and various biotechnological and biomedical applications of membrane proteins.