新生儿Fc受体(FcRn)是负责免疫球蛋白G(IgG)跨细胞双向运输的受体,维持血清中的IgG水平,并协助抗原呈递。不幸的是,对马匹的FcRn知之甚少。因此,这项研究的目的是提供有关FcRn在马组织中位置的基本信息。从六匹混合品种的马身上收集了组织,年龄,安乐死后立即发生性行为。采样位置包括呼吸道,胃肠道(GIT),其他内脏器官,角膜,窒息和腕关节的滑膜。进行组织学分析的组织是固定的,横截面,并为FcRn染色。捕获感兴趣的区域,并用表示为相对荧光(RF)的数据进行分析,以指示FcRn丰度。将用于qPCR分析的组织置于RNAlater中,并使用2-ΔΔCT方法计算FcRn转录本(FCGRT)的相对定量(RQ)。归一化为三个参考基因的几何平均值(ACTB,GADPH,HPRT1)。使用SAS的一般线性模型程序分析数据。通过免疫荧光和qPCR分析,FcRn的丰度在组织类型之间存在差异(P<0.01)。关节滑膜和呼吸道组织的RF最高,GIT组织表达中度RF,其他内脏器官的RF最低。相反,肝脏和肾脏组织的RQ最高,而胃和角膜的RQ最低。这些数据为未来有关马的FcRn和IgG及其在疾病预防和治疗中的作用的研究奠定了基础。
The neonatal Fc receptor (
FcRn) is the receptor responsible for bidirectional transport of immunoglobulin G (IgG) across cells, maintenance of IgG levels in serum, and assisting with antigen presentation. Unfortunately, little is known about
FcRn in horses. Therefore, the objective of this study was to provide fundamental information regarding the location of FcRn in equine tissues. Tissues were collected from six horses of mixed breed, age, and sex immediately following euthanasia. Sampling locations included the respiratory tract, gastrointestinal tract (GIT), other visceral organs, cornea, and synovial membrane of the stifle and carpal joints. Tissues for histological analysis were fixed, cross sectioned, and stained for
FcRn. Areas of interest were captured and analyzed with data represented as relative fluorescence (RF) to indicate FcRn abundance. Tissues for qPCR analysis were placed in RNAlater and relative quantification (RQ) of
FcRn transcripts (FCGRT) was calculated using the 2-ΔΔCT method, normalized to the geometric mean of three reference genes (ACTB, GADPH, HPRT1). Data were analyzed using the general linear model procedure of SAS. Abundance of
FcRn differed between tissue types by immunofluorescence and qPCR analysis (P < 0.01). Joint synovium and respiratory tract tissues had the highest RF, GIT tissues expressed moderate RF, and other visceral organs had the lowest RF. Conversely, liver and kidney tissues had the highest RQ while the stomach and cornea had the lowest RQ. These data lay the foundation for future studies regarding FcRn and IgG in horses and their roles in disease prevention and treatment.