Achilleafragranantissima是一种灌木植物,属于阿拉伯和埃及的菊科科。它被用作民间药物,是酚酸的良好来源,黄酮类化合物,和一些活性化合物。目的:探讨紫草对乳腺癌MCF-7细胞的抗癌作用及其与细胞凋亡的关键机制。研究了香花乙醇提取物的毒性和药代动力学研究,以了解抗乳腺癌的特性。反过来,通过MTT方法获得细胞毒性和细胞活力。此外,锥虫蓝排除和显微镜检查证明存在凋亡细胞。再一次,荧光染色,如AO/EtBr,DCFH-DA,Rho-123和Hoechst-33342揭示了紫花苜蓿效应的细胞细胞质纪律。此外,细胞功能测试,如伤口愈合,菌落形成,并证明了Transwell侵袭试验。此外,qRT-PCR技术验证了香菇诱导的凋亡网络基因(Caspase-3,Caspase-8,Caspase-9,细胞色素c,BCL-2,BID,巴克斯,PARP,PTEN,PI3K,和Akt)表达进行评价。主要是,免疫印迹技术证明了凋亡蛋白的表达水平,如切割的PARP,CYCS,和FADD。该研究证实,在MCF-7细胞中,A.fragrantissima在20μg/mL下发挥细胞毒性24小时。此外,降低细胞活力,产生凋亡细胞和受损的细胞表面与死物质。因此,它创造了ROS物种的积累,线粒体膜电位的丧失,和MCF-7细胞中DNA的片段化。此外,它阻止细胞迁移,诱导菌落形成能力丧失,并抑制细胞入侵。此外,A.fragrantissima显着上调基因,例如caspase-3,9,细胞色素c,BID,巴克斯,和PTEN,同时下调Pi3K/Akt信号。尽管如此,A.fragrantissima诱导裂解的PARP,CYCS,和MCF-7细胞中的FADD蛋白有助于细胞凋亡。
Achillea fragrantissima is a shrub plant that belongs to the Asteraceae family in Arabia and Egypt. It is used as folk medicine and is a good source of phenolic acids, flavonoids, and some active compounds. To investigate the anti-cancer effect of A.fragrantissima on breast cancer MCF-7 cells and find the critical mechanism involved in apoptosis. The toxicity and pharmacokinetic studies of ethanolic extract of A.fragrantissima was examined for anti-breast cancer properties. In turn, cytotoxicity and cell viability were achieved by the MTT method. Furthermore, the trypan blue exclusion and microscopy examination proved the presence of apoptotic cells. Again, fluorescent staining such as AO/EtBr, DCFH-DA, Rho-123, and Hoechst-33342 reveals the cellular cytoplasmic disciplines upon A. fragrantissima effect. Moreover, cellular functioning tests like wound healing, colony formation, and Transwell invasion assay were demonstrated. In addition, the qRT-PCR technique authenticates the A. fragrantissima -induced apoptotic network genes (Caspase-3, Caspase-8, Caspase-9, Cytochrome c, BCL-2, BID, BAX, PARP, PTEN, PI3K, and Akt) expression were evaluated. Mainly, the Immunoblot technique proved the expressed level of apoptotic proteins such as cleaved PARP, CYCS, and FADD. This study confirmed that the A. fragrantissima exerts cytotoxicity at 20 μg/mL for 24 hrs in MCF-7 cells. Also, decreases cellular viability, producing apoptotic cells and damaged cellular surfaces with dead matter. Consequently, it creates ROS species accumulation, loss of mitochondrial membrane potential, and fragmentation of DNA in MCF-7 cells. Furthermore, it arrests cell migration, induces colony-forming ability loss, and suppresses cell invasion. In addition, A. fragrantissima significantly upregulates genes such as caspase-3, 9, cytochrome c, BID, BAX, and PTEN while downregulating the Pi3K/ Akt signaling. Nonetheless, A.fragrantissima induced cleaved PARP, CYCS, and FADD proteins in MCF-7 cells to avail apoptosis.