Pollution of water resources with nitrate is currently one of the major challenges at the global level. In order to make macro-policy decisions in water safety plans, it is necessary to carry out nitrate risk assessment in underground water, which has not been done in Fars province for all urban areas. In the current study, 9494 drinking water samples were collected in four seasons in 32 urban areas of Fars province in Iran, between 2017 and 2021 to investigate the non-carcinogenic health risk assessment. Geographical distribution maps of hazard quotient were drawn using geographical information system software. The results showed that the maximum amount of nitrate in water samples in 4% of the samples in 2021, 2.5% of the samples in 2020 and 3% of the samples in 2019 were more than the standard declared by World Health Organization guidelines (50 mg/L). In these cases, the maximum amount of nitrate was reported between 82 and 123 mg/L. The HQ values for infants did not exceed 1 in any year, but for children (44% ± 10.8), teenagers (10.8% ± 8.4), and adults (3.2% ± 1.7) exceeded 1 in cities, years, and seasons, indicating that three age groups in the studied area are at noticeably significant non-carcinogenic risk. The results of the Monte Carlo simulation showed that the average value of non-carcinogenic risk was less than 1 for all age groups. Moreover, the maximum HQ values (95%) were higher than 1 for both children and teenager, indicating a significant non-carcinogenic risk for the two age groups.

Social capital is an essential type of capital that influences the growth and development of societies. The present descriptive-survey research aimed to capture CEOs\' social capital predictors in the agricultural consultation, technical, and engineering service companies in Fars province, Iran. The CEOs, who amounted to 107 people, all participated in the research. The data collection instrument was a questionnaire whose content and face validity were confirmed by a panel of experts and whose reliability was calculated by Cronbach\'s alpha at 0.82. Data were analyzed in the SPSS22 software package. Based on data analysis, eight social capital items were derived and prioritized. They included social participation, social proactivity, social trust, neighborhood connections, friends and family connections, capacity to accept differences, appreciation of life, and work connections. Based on the ranking of these elements, social proactivity, work connections, and friends and family connections were ranked first to third, respectively. Also, step-by-step multiple regression analysis revealed that the three variables of the feeling of job security, investment, and media were the independent variables that accounted for the CEO\'s social capital. Programs provided by the media should focus on promoting people\'s social solidarity. Some investment must be made by these companies in social activities and encouragement of the target community\'s participation and trust. The success of the agricultural consultation, technical, and engineering service companies is based on the principles of specialty, trust, participation, and social solidarity, showing the existence of social capital in these companies. Therefore, social capital and factors that predict it influence the productivity and efficiency of the companies.

UNASSIGNED: Lung cancer (LC) is the second most common and deadliest cancer in the world. Despite the control of the progressive course of LC in developed countries, studies indicate an increase in the incidence of the disease in developing countries. We designed a stepwise approach-based surveillance system for registering LC in our region (fars lung cancer registry \"FaLCaRe\" Project).
UNASSIGNED: A questionnaire was designed and agreed upon by the steering committee using the Delphi method. Variables in nine fields were divided into three groups based on their importance: core, expanded core, and optional. The web-based data bank software was designed. The informative site about LC and team services was designed and launched for professional and community (www.falcare.org) educational purposes.
UNASSIGNED: 545 variables in nine fields were designed (20 core variables). Primary data of 39 LC patients (24 men and 15 women) with a mean age of 62 years were analyzed. Twenty-six patients had a history of smoking. Moreover, 39% and 26% of patients had a history of hookah smoking and opium use, respectively. Adenocarcinoma was the most prevalent pathologic findings in cases. More than 80% of patients were diagnosed in stages 3 and 4 of cancer.
UNASSIGNED: FaLCaRe Project with the capabilities seen in it can be used as a model for national LC registration. With continuous valid data registry about LC, it is possible to make decisions at the national level for control and management its consequences while drawing the natural history of the LC.

Canine visceral leishmaniasis (CVL) is endemic in the southern parts of Iran. The current study aimed at molecular and serological evaluation of zoonotic visceral leishmaniasis in dogs in Fars province, southern Iran. Blood samples were collected from 60 dogs in the three villages in Fars Province. Serum samples were tested for antibodies against L. infantum by direct agglutination test (DAT). DNA was extracted from each dog\'s buffy coat and tested by PCR, targeting the Leishmania ITS-2 region. From a total of 60 studied dogs, 25 (41.7%) were female, and 35 (58.3%) were male. Dogs\' age ranged from 1 to 7 years, with a mean age of 2.97 (±1.4) years. Anti-Leishmania antibodies were detected in sera samples of 28 (46.7%) dogs, (titre ≥ 1:320). Out of 28 seropositive cases, 13 (46.4%) were female, and 15 (53.6%) were male. Association between seropositivity and dogs\' clinical signs was statistically significant (p < .05). Leishmania DNA was detected in the buffy coat of 3 of 60 studied dogs which were all seropositive by DAT. The PCR products were sequenced and molecular analysis showed that two of the isolates were Leishmania infantum, and one was L. tropica. The high proportion of seropositive dogs indicates the important role of these animals in the epidemiology of the disease in the region. Infected dogs with or without signs, especially those that are molecularly positive, can act as an active reservoir of the disease in the area.

HIV-infected patients have immunological and clinical features that might affect the pathogenesis, as well as the outcome of the HIV/HEV co-infection. The current study aimed to determine the seroprevalence of anti-HEV antibodies and HEV antigens among HIV-infected patients in Fars Province, Southwest Iran.
Blood samples (5 mL) were collected from 251 HIV-confirmed patients. Respective data, including patients\' demographic information, were obtained for each patient. The presence of HEV antigens and anti-HEV antibodies (IgG) were assessed by commercial ELISA kits, based on the manufacturers\' instructions.
Out of 251 cases, 158 (62.9%) were male and 91 (36.3%) were female. Patients\' age varied from 14 to 83 (mean: 40 ± 9.7) years. Out of 251 HIV positive cases, 26 (10.4%) were positive for anti-HEV IgG antibodies and 6 (2.4%) were positive for HEV-antigens. Also, 2 (0.8%) of the patients were positive for both anti-HEV IgG antibodies and antigens. Statistical analysis revealed no significant association between sex and seropositivity to either HEV antigen or antibodies. Moreover, no significant association was seen between age and seropositivity to HEV antigen or antibody (P = 0.622 and 0.945, respectively).
Our results showed a relatively low prevalence of HEV-antibodies in HIV-infected patients, indicating that HIV positive patients may not be at greater risk of HEV infection than the general population. Moreover, HEV-antigen was detected in a few cases of HIV-infected individuals which indicate an acute or chronic HEV infection in these patients.

The present study aimed to find out the levels of anti-HBsAb among vaccinated children in a rural community in Fars Province, Southern Iran. Blood samples were taken from 550 children, aged 1-12 years (mean 6.4 ± 3.5), in 2017 from three villages in the area. A structured questionnaire was used to get the sociodemographic data of the subjects along with determinants concerning the Hepatitis B. Sera samples were examined for anti-HBsAb, using an ELISA commercial kit. Anti-HBsAb were detected in 468 (85.1%) of the subjects. Of the seropositive subjects, 37 (45.1%) were female and 45 (54.9%) were male. In the age group of 0-5 years, 88.7% of the subjects were seropositive. This rate was 84.3% and 78.1% in the age group of 6-10 years old and older than 10 years, respectively. There was a significant association (p < .05) between the anti-HBsAb and age. Findings of the current study revealed that children living in a rural community in southern Iran have appropriate protection against HBV even more than 10 years after being vaccinated. The decline in seropositivity rate of anti-HBsAb with age may further point out the need for a booster dose of HBV vaccine.

The purpose of the present study was to isolate and identify the pathogenic agents in Acipenser stellatus (Pallas, 1771) and Huso huso, (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemia symptoms such as decreasing of appetite, unbalanced swimming, expanded wounds, and petechia on the body surfaces, pectoral fins rot, visceral hemorrhage, bleeding on the spleen, and heart ascites were collected. Then samples were cultured on brain heart infusion agar growth media, stain and biological and biochemical tests on purified bacteria were performed. On the other hand, 16S rDNA region of the isolated organism was amplified using PCR. The amplified gene fragment was sequenced and evolutionary history was inferred by phylogenetic tree construction using neighbor-joining method. Results indicated that two bacterial species including Chryseobacteriumjoostei which isolated from the kidney of stellate sturgeon (43.00%), and Aeromonasveronii which isolated from the spleen of both sturgeon species (75.00% and 31.00% from beluga and stellate sturgeon, respectively), were recognized. Phylogenetic tree analysis showed that Fars isolated organisms including A. veronii and C. joostei had highest similarity with A. veronii bv veronii and C. joostei isolated from France, respectively.

Fluoride is a natural element among minerals, geochemical sediments and natural water systems which is entered to body chain by drinking water. Groundwater is the main and the best source of drinking water in southern areas of Iran especially in the cities of Lar and Gerash (Fars province). So due to the health significance fluoride including dental and skeletal fluorosis, fertility, abortion and thyroid diseases, etc., measuring has high importance in the water resources of this region of Iran. Fluoride concentration was 0.35-3.46 mg/L and 78.26% drinking water sources contains fluoride concentration above the WHO guideline.

Cystic echinococcosis (CE) is one of the most important zoonotic diseases in different geographical areas of the world including Iran. The current study aimed to assess the seroprevalence of human cystic echinococcosis (CE) in healthy blood donors in Fars province, southern Iran. A total of 1068 serum samples were collected from blood donors from five blood service centers of Fars province in south of Iran. Antigen B was prepared from sheep hydatid cyst fluid and collected sera were evaluated for anti-hydatid cyst antibodies, using antigen-B ELISA. Demographic features of the participants were also recorded during the sample collection. Anti hydatid cyst antibodies were detected in sera of 60 out of 1068 blood donors corresponding to overall seroprevalence of 5.6% in this population. Rate of seroprevalence was 6.7% in females and 5.5% in males. The highest rate of infection (8.3%) was found in age group of higher than 50 years old. There were no significant differences between seropositivity to CE and sex, age or place of residence of the participants (P > 0.05). Findings of this study showed that the rate of CE infection in Fars province, southern Iran, is relatively high. Some of these seropositive cases may have active hydatid cyst in their tissues without overt sign or symptoms.

UNASSIGNED: Cystic echinococcosis (CE), known as hydatid cyst, is a zoonotic parasitic infection caused by the larval stage of Echinococcus granulosus (E. granulosus). Antigen B, the major component of hydatid cyst fluid, is encoded by members of a multigene family. The present study aimed to evaluate the genetic diversity of the gene encoding antigen B8/1 (EgAgB8/1) among the main intermediate hosts of E. granulosus.
UNASSIGNED: Twenty-eight hydatid cyst isolates (10 sheep, 9 human, and 9 cattle) were collected in Fars province, Iran. DNA was extracted from each cyst and PCR, followed by DNA sequencing was used to identify potential EgAgB8/1 sequence variation and polymorphism. A phylogenetic tree was constructed using MEGA 7.0 software and the maximum likelihood method.
UNASSIGNED: Using EgAgB8/1 primers, an approximately 315 bp band was amplified from all the isolates. The PCR products were sequenced, and the sequences were deposited in GenBank (accession numbers, KY709266-KY709293). The polymorphism variation among the isolates was 0.0, while intra-species variation within the isolates and related sequences in GenBank was 0.5-1%. Analysis of the phylogenetic tree revealed that the isolates from humans, sheep, and cattle all cluster in one group and are homologous to the EgAgB8/1 M1 allele.
UNASSIGNED: Findings of this study revealed close similarity between the EgAgB8/1 of human, sheep, and cattle E. granulosus isolates. However, differences were found between the EgAgB8/1 sequences in our study and those reported from other CE endemic areas. Whether such similarities and differences exist in other subunits AgB subunits require further study.