普氏粪杆菌是人体肠道中最重要的丁酸产生菌之一。以前的研究表明存在几个系统发育群体,由于物种水平的差异,在物种中,因此,分类学的重新评估对于进一步了解重要的人类共生体的生态学至关重要。在这里,我们检查表型,生理,6株普氏F.prausnitzii菌株的化学分类学和系统基因组特征(BCRC81047T=ATCC27768T,A2-165T=JCM31915T,APC918/95b=JCM39207,APC942/30-2=JCM39208,APC924/119=JCM39209和APC922/41-1T=JCM39210T)保藏在公共培养物集合中,并带有两个参考菌株丁氏杆菌属JCM39212T和长粪杆菌JCM39211T。粪杆菌。还包括从肉鸡盲肠中分离的JCM17207T。三个菌株(BCRC81047T,JCM39207和JCM39209)共享超过96.6%的平均核苷酸同一性(ANI)和69.6%的数字DNA-DNA杂交(dDDH)值,表明这三个菌株是同一物种的成员。另一方面,其余三株普劳斯尼齐(JCM31915T,根据ANI和dDDH值,JCM39208和JCM39210T)与上述三个菌株明显分开。相反,JCM39208显示ANI和dDDH值超过了F.LongumJCM39211T的物种歧视的截止值(>70%dDDH和>95-96%ANI),而JCM31915T,JCM39210T和JCM17207T没有与任何测试菌株共享超过当前接受的截止值的dDDH和ANI值,包括其中。此外,这些菌株的细胞脂肪酸模式与其他F.prausnitzii菌株略有不同。根据收集的数据,F.prausnitziiJCM31915T,F.prausnitziiJCM39210T和Faecalibacteriumsp。JCM17207T代表了粪杆菌属的三个新物种,为此命名为DuncaniaeFaecalibacteriumsp。11月。(型应变JCM31915T=DSM17677T=A2-165T),哈氏粪杆菌。11月。(类型菌株JCM39210T=DSM107841T=APC922/41-1T)和鸡粪杆菌。11月。(型应变JCM17207T=DSM23680T=ic1379T)提出。
Faecalibacterium prausnitzii is one of the most important butyrate-producing bacteria in the human gut. Previous studies have suggested the presence of several phylogenetic groups, with differences at the species level, in the species, and a taxonomic re-evaluation is thus essential for further understanding of ecology of the important human symbiont. Here we examine the phenotypic, physiological, chemotaxonomic and phylogenomic characteristics of six F. prausnitzii strains (BCRC 81047T=ATCC 27768T, A2-165T=JCM 31915T, APC918/95b=JCM 39207, APC942/30-2=JCM 39208, APC924/119=JCM 39209 and APC922/41-1T=JCM 39210T) deposited in public culture collections with two reference strains of Faecalibacterium butyricigenerans JCM 39212T and Faecalibacterium longum JCM 39211T. Faecalibacterium sp. JCM 17207T isolated from caecum of broiler chicken was also included. Three strains of F. prausnitzii (BCRC 81047T, JCM 39207 and JCM 39209) shared more than 96.6 % average nucleotide identity (ANI) and 69.6 % digital DNA-DNA hybridization (dDDH) values, indicating that the three strains are members of the same species. On the other hand, the remaining three strains of F. prausnitzii (JCM 31915T, JCM 39208 and JCM 39210T) were clearly separated from the above three strains based on the ANI and dDDH values. Rather, JCM 39208 showed ANI and dDDH values over the cut-off values of species discrimination (>70 % dDDH and >95-96 % ANI) with F. longum JCM 39211T, whereas JCM 31915T, JCM 39210T and JCM 17207T did not share dDDH and ANI values over the currently accepted cut-off values with any of the tested strains, including among them. Furthermore, the cellular fatty acid patterns of these strains were slightly different from other F. prausnitzii strains. Based on the collected data, F. prausnitzii JCM 31915T, F. prausnitzii JCM 39210T and Faecalibacterium sp. JCM 17207T represent three novel species of the genus Faecalibacterium, for which the names Faecalibacterium duncaniae sp. nov. (type strain JCM 31915T=DSM 17677T=A2-165T), Faecalibacterium hattorii sp. nov. (type strain JCM 39210T=DSM 107841T=APC922/41-1T) and Faecalibacterium gallinarum sp. nov. (type strain JCM 17207T=DSM 23680T=ic1379T) are proposed.