通过串扰,FLAGELLINSensitive2(FLS2)和呼吸性爆发氧化酶同型D(RBOHD)参与调节细胞活性氧(ROS)的稳态,并与植物对生物和非生物胁迫的代谢反应有关。在本研究中,我们研究了拟南芥幼苗在干旱和盐条件下的代谢组,以更好地了解FLS2和RBOHD依赖性信号在调节非生物胁迫反应中的潜在作用。我们确定了由FLS2和RBOHD调节的常见代谢物和基因,并参与了对干旱和盐胁迫的反应。在干旱条件下,D-天冬氨酸和相关基因的表达,如天冬氨酸合成酶2(ASN2),在fls2和rombed/f双突变体中均增加。氨基酸的积累,碳水化合物,和荷尔蒙,比如L-脯氨酸,D-核糖,在盐条件下,fls2和rbohd/f双突变体中吲哚乙醛都增加,相关基因的表达也是如此,如PROLINEIMOPTIDASE,磷酰焦磷酸盐合成酶5和硝化酶3。总的来说,这些结果表明,FLS2-RBOHD模块通过调节代谢产物的积累和代谢产物合成相关基因的表达,通过ROS信号调节植物对干旱和盐胁迫的反应。
Through crosstalk, FLAGELLIN SENSITIVE 2 (
FLS2) and RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) are involved in regulating the homeostasis of cellular reactive oxygen species (ROS) and are linked to the metabolic response of plants toward both biotic and abiotic stress. In the present study, we examined the metabolome of Arabidopsis seedlings under drought and salt conditions to better understand the potential role of
FLS2 and RBOHD-dependent signaling in the regulation of abiotic stress response. We identified common metabolites and genes that are regulated by
FLS2 and RBOHD, and are involved in the response to drought and salt stress. Under drought conditions, D-aspartic acid and the expression of associated genes, such as ASPARAGINE SYNTHASE 2 (ASN2), increased in both fls2 and robed/f double mutants. The accumulation of amino acids, carbohydrates, and hormones, such as L-proline, D-ribose, and indoleacetaldehyde increased in both
fls2 and rbohd/f double mutants under salt conditions, as did the expression of related genes, such as PROLINE IMINOPEPTIDASE, PHOSPHORIBOSYL PYROPHOSPHATE SYNTHASE 5, and NITRILASE 3. Collectively, these results indicate that the
FLS2-RBOHD module regulates plant response to drought and salt stress through ROS signaling by adjusting the accumulation of metabolites and expression of genes related to metabolite synthesis.