目的:远北地区土著居民的免疫反应水平可能取决于气候环境的极端不适因素和人口的遗传特征。因此,这项研究的目的是确定居住在远北(摩尔曼斯克地区)的KolaSaami和俄罗斯人的免疫状态的独特因素。
方法:对外周静脉血成分进行了复杂的免疫学研究,涉及67名萨米人和81名21-55岁的远北(摩尔曼斯克地区)俄罗斯居民。早晨空腹从肘静脉收集血液。对于外周静脉血,我们获得了中性粒细胞的血象和吞噬活性,通过间接免疫过氧化物酶反应和使用EpicsXL仪器(BeckmanCoulter,美国),获得的IL-6,IFN-γ,IL-10,IgA,M,G,E,sCD54和sCD62L,转铁蛋白,和sCD71通过酶免疫测定,使用来自Bio-RAD(德国)的“Evolis”酶免疫测定分析仪,并通过使用3.5%沉淀获得循环免疫复合物,4.0%,和7.5%PEG-6000。在早晨唾液中测定sIgA含量,尿液,和胃肠道分泌物.结果采用描述性统计分析,独立样本t准则,和皮尔逊相关系数。
结果:萨米族人白细胞总数的减少与白细胞减少症的高发率有关,淋巴细胞减少,和中性粒细胞减少症.显示活性吞噬细胞和血清IgA和sIgA浓度缺乏,CD4和CD8受体淋巴细胞含量增加,膜和游离粘附分子,和转铁蛋白。白细胞增多,淋巴细胞增多,嗜中性粒细胞增多症,单核细胞增多,和嗜酸性粒细胞增多,自然杀伤细胞含量增加,IL-6和IL-10在俄国人中记录更频繁。高水平的IgE和IFN-γ,它们具有最明显的细胞毒性,表明免疫机制紧张,是萨米人和俄罗斯人的特征。
结论:KolaSaami免疫状态的独特方面与活性吞噬细胞和血清IgA和sIgA浓度的不足以及细胞毒性淋巴细胞含量的增加有关,细胞间粘附分子,转铁蛋白,和IFN-γ促炎细胞因子。远北地区的俄罗斯居民的特征是适应性反应,表现为抗体依赖性细胞毒性的增加,涉及IL-6和IL-10。
The level of immunological reactivity of the indigenous inhabitants of the Far North may depend on the extreme-discomfort factors of the climatic environment and genetic traits of the population. Therefore, the aim of this study was to identify the distinctive elements of the immune status of the Kola Saami and Russians living in the Far North (Murmansk Region).
A complex immunological study of peripheral venous blood composition was performed and involved 67 Saami and 81 Russian inhabitants of the Far North (Murmansk Region) who were aged 21-55. Blood was collected from the cubital vein on an empty stomach in the morning. For the peripheral venous blood, we obtained a hemogram and phagocytic activity of neutrophils, determined the content of lymphocytes with CD4, CD8, CD16, and CD71 phenotypes by indirect immunoperoxidase reaction and flow cytometry using an Epics XL apparatus (Beckman Coulter, United States), obtained concentrations of IL-6, IFN-γ, IL-10, IgA, M, G, E, sCD54, and sCD62L, transferrin, and sCD71 by enzyme immunoassay using the \"Evolis\" enzyme immunoassay analyzer from Bio-RAD (Germany), and obtained circulating immune complexes by precipitation using 3.5%, 4.0%, and 7.5% PEG-6000. The sIgA content was determined in morning saliva, urine, and gastrointestinal tract secretions. The results were analyzed using descriptive statistics, independent sample t-criterion, and Pearson correlation coefficient.
A decrease in the total number of leukocyte cells in the Saami was associated with a high incidence of leukopenia, lymphopenia, and neutropenia. Deficiency in active phagocytes and serum IgA and sIgA concentrations was revealed as well as increased contents of CD4+ and CD8+ receptor lymphocytes, membrane and free adhesion molecules, and transferrin. Leukocytosis, lymphocytosis, neutrophilia, monocytosis, and eosinophilia with increased contents of natural killer cells, IL-6, and IL-10 were recorded more frequently in the Russians. High levels of IgE and IFN-γ, which have the most pronounced cytotoxicity and are indicative of strained immune mechanisms, were characteristic of the Saami and Russians.
The distinctive aspects of the immune status of the Kola Saami is related to the deficit in the active phagocyte and serum IgA and sIgA concentrations as well as the increase in the contents of cytotoxic lymphocytes, intercellular adhesion molecules, transferrin, and IFN-γ proinflammatory cytokines. The Russian inhabitants of the Far North are characterized by an adaptive response manifested by an increase in antibody-dependent cytotoxicity, involving IL-6 and IL-10.