肾盂肾炎相关菌毛(P菌毛)是肠外致病性大肠杆菌菌株(ExPECs)的最公认的粘附决定因素之一。已经描述了编码菌毛主要结构亚基PapA的基因的十二种变体,以及编码粘附素亚基PapG的基因的三种变体。然而,它们在ExPEC多样性中的分布尚未得到全面解决。这种分布的完整景观可能对于描述有关ExPEC致病性机制的基础研究以及跟踪ExPEC谱系的进化是有价值的。特别是那些与流行病学最相关的。因此,我们进行了大量描述性研究,以检测NCBIAssemblyRefseq数据库中包含的基因组序列所代表的不同大肠杆菌基因型的papA和papG变异.最常见的papA变体是F11,F10,F48,F16,F12和F7-2,它们与最相关的ExPEC基因型显着相关。系统群B2和D,以及序列类型ST95、ST131、ST127、ST69、ST12和ST73。另一方面,papGII变体是迄今为止最常见的,其次是papGIII,还发现两者都与常见的ExPEC基因型有显著关联。我们注意到基因组的存在,主要属于序列类型ST12,具有两个或三个papA变体和两个papG变体。此外,最常见的papA和papG变体也在代表从人类和动物如家禽分离的菌株的记录中检测到,牛,还有狗,支持先前潜在交叉传输的假设。最后,我们对智利尿路致病性大肠杆菌菌株的17个基因组进行了鉴定,发现ST12和ST73是主要的序列类型。检测到变体F7-1、F7-2、F8、F9、F11、F13、F14、F16和F48的papA,并检测到papG的papGII和papGIII变异。在该集合中,在19例papA变体中的16例和9例papG变体中的7例中,还发现了与NCBIAssemblyRefseq数据库中包含的基因组分析中观察到的序列类型的显着关联。这种全面的表征可能支持有关菌毛介导的ExPEC粘附性的未来基础研究以及未来的分型或流行病学研究,以监测产生菌毛的ExPEC的进化。
The pyelonephritis-associated fimbria (P fimbria) is one of the most recognized adhesion determinants of extraintestinal pathogenic Escherichia coli strains (ExPECs). Twelve variants have been described for the gene encoding the P fimbria major structural subunit PapA and three variants for the gene encoding the adhesin subunit PapG. However, their distribution among the ExPEC diversity has not been comprehensively addressed. A complete landscape of that distribution might be valuable for delineating basic studies about the pathogenicity mechanisms of ExPECs and following up on the evolution of ExPEC lineages, particularly those most epidemiologically relevant. Therefore, we performed a massive descriptive study to detect the papA and papG variants along different E. coli genotypes represented by genomic sequences contained in the NCBI Assembly Refseq database. The most common papA variants were F11, F10, F48, F16, F12, and F7-2, which were found in significant association with the most relevant ExPEC genotypes, the phylogroups B2 and D, and the sequence types ST95, ST131, ST127, ST69, ST12, and ST73. On the other hand, the papGII variant was by far the most common followed by papGIII, and both were also found to have a significant association with common ExPEC genotypes. We noticed the presence of genomes, mainly belonging to the sequence type ST12, harboring two or three papA variants and two papG variants. Furthermore, the most common papA and papG variants were also detected in records representing strains isolated from humans and animals such as poultry, bovine, and dogs, supporting previous hypotheses of potential cross-transmission. Finally, we characterized a set of 17 genomes from Chilean uropathogenic E. coli strains and found that ST12 and ST73 were the predominant sequence types. Variants F7-1, F7-2, F8, F9, F11, F13, F14, F16, and F48 were detected for papA, and papGII and papGIII variants were detected for papG. Significant associations with the sequence types observed in the analysis of genomes contained in the NCBI Assembly Refseq database were also found in this collection in 16 of 19 cases for papA variants and 7 of 9 cases for the papG variants. This comprehensive characterization might support future basic studies about P fimbria-mediated ExPEC adherence and future typing or epidemiological studies to monitor the evolution of ExPECs producing P fimbria.