出芽酵母中减数分裂的开始不会使细胞发生减数分裂。因此,两种不同的信号级联可能差异调节出芽酵母的减数分裂起始和定型。为了区分这些信号级联的作用,我们重建了减数分裂起始和定型过程中具有上调基因的蛋白质-蛋白质相互作用网络和基因调控网络.分析综合网络,我们确定了四个主监管机构(MR)[Ume6p,msn2p,Met31p,Ino2p],三个转录因子(TFs),和279个针对减数分裂起始独特的靶基因(TG),和三个MR[Ndt80p,Aro80p,Rds2p],11个TFs,和948个减数分裂承诺特有的TG。减数分裂起始和承诺的转录级联中这些不同成员的功能富集分析表明,营养线索重新连接基因表达以启动减数分裂和染色体重组使细胞进入减数分裂。由于减数分裂染色体重组在真核生物中高度保守,我们比较了酿酒酵母两个减数分裂阶段的转录级联中独特成员与子囊门成员的进化率,揭示了在减数分裂期间控制染色体重组的转录级联经历了更大的纯化选择压力(对于Ashbyagossypii,P值=0.0013、0.0382、0.0448、0.0369、0.02967、0.04937、0.03046、0.03357和<0.00001,Yarrowialipolytica,汉森德巴酵母,烟曲霉,粗糙神经孢子菌,乳酸克鲁维酵母,蓬布裂殖酵母,冷冻裂殖酵母,和八孢裂殖酵母,分别)。这项研究划分了驱动减数分裂开始和承诺的关键参与者,并证明了他们在出芽酵母中的不同进化率。
Initiation of meiosis in budding yeast does not commit the cells for meiosis. Thus, two distinct signaling cascades may differentially regulate meiosis initiation and commitment in budding yeast. To distinguish between the role of these signaling cascades, we reconstructed protein-protein interaction networks and gene regulatory networks with upregulated genes in meiosis initiation and commitment. Analyzing the integrated networks, we identified four master regulators (MRs) [Ume6p, Msn2p, Met31p, Ino2p], three transcription factors (TFs), and 279 target genes (TGs) unique for meiosis initiation, and three MRs [Ndt80p, Aro80p, Rds2p], 11 TFs, and 948 TGs unique for meiosis commitment. Functional enrichment analysis of these distinct members from the transcriptional cascades for meiosis initiation and commitment revealed that nutritional cues rewire gene expression for initiating meiosis and chromosomal recombination commits cells to meiosis. As meiotic chromosomal recombination is highly conserved in eukaryotes, we compared the evolutionary rate of unique members in the transcriptional cascade of two meiotic phases of Saccharomyces cerevisiae with members of the phylum Ascomycota, revealing that the transcriptional cascade governing chromosomal recombination during meiosis commitment has experienced greater purifying selection pressure (P value = 0.0013, 0.0382, 0.0448, 0.0369, 0.02967, 0.04937, 0.03046, 0.03357 and < 0.00001 for Ashbya gossypii, Yarrowia lipolytica, Debaryomyces hansenii, Aspergillus fumigatus, Neurospora crassa, Kluyveromyces lactis, Schizosaccharomyces pombe, Schizosaccharomyces cryophilus, and Schizosaccharomyces octosporus, respectively). This study demarcates crucial players driving meiosis initiation and commitment and demonstrates their differential rate of evolution in budding yeast.