这项研究旨在检查用eldecalcitol(ELD)进行卵巢切除的大鼠长骨的骨phy骨和干phy端之间的基于最小化的骨形成。将16周龄雌性大鼠分为四组:接受赋形剂的假手术大鼠(假手术组),接受媒介物的去卵巢(OVX)大鼠(媒介物组),或ELD(30或90纳克/千克体重,分别;ELD30和ELD90组)。ELD给药增加了骨体积和小梁厚度,减少OVX大鼠的骨phy和干mis端破骨细胞的数量。Sham和Vehicle组在两个区域中主要表现出基于重塑的骨形成。ELD组的骨phy显示出基于最小化的骨形成的频率明显高于基于重塑的骨形成。相比之下,与ELD30组相比,ELD90组的干干meta端表现出显著更多的基于最小化的骨形成.然而,在ELD90组中,基于最小模型的骨形成和基于重塑的骨形成之间没有显着差异。虽然小模型诱导的新骨含有很少的硬化蛋白免疫反应性骨细胞,潜在的预先存在的骨头有很多。ELD组的骨phy中,在小型骨诱导的骨中,硬化素阳性骨细胞的百分比显着降低,而在干mis端则没有降低。因此,似乎ELD可能会在骨phy中而不是在干phy端中诱导基于最小化的骨形成,并且ELD驱动的最小化可能与硬化素合成的抑制有关。
This study aimed to examine minimodeling-based bone formation between the
epiphyses and metaphyses of the long bones of eldecalcitol (ELD)-administered ovariectomized rats. Sixteen-week-old female rats were divided into four groups: sham-operated rats receiving vehicle (Sham group), ovariectomized (OVX) rats receiving vehicle (Vehicle group), or ELDs (30 or 90 ng/kg BW, respectively; ELD30 and ELD90 groups). ELD administration increased bone volume and trabecular thickness, reducing the number of osteoclasts in both the
epiphyses and metaphyses of OVX rats. The Sham and Vehicle groups exhibited mainly remodeling-based bone formation in both regions. The
epiphyses of the ELD groups showed a significantly higher frequency of minimodeling-based bone formation than remodeling-based bone formation. In contrast, the metaphyses exhibited significantly more minimodeling-based bone formation in the ELD90 group compared with the ELD30 group. However, there was no significant difference between minimodeling-based bone formation and remodeling-based bone formation in the ELD90 group. While the minimodeling-induced new bone contained few sclerostin-immunoreactive osteocytes, the underlying pre-existing bone harbored many. The percentage of sclerostin-positive osteocytes was significantly reduced in the minimodeling-induced bone in the
epiphyses but not in the metaphyses of the ELD groups. Thus, it seems likely that ELD could induce minimodeling-based bone formation in the
epiphyses rather than in the metaphyses, and that ELD-driven minimodeling may be associated with the inhibition of sclerostin synthesis.