秸秆中木质素的复杂结构使得水解具有挑战性,使其成为当前研究的重点。然而,关于酶诱导剂(MnSO4)与功能微生物结合对秸秆堆肥过程中木质素降解的影响的研究有限。基于此,本研究设立了四个堆肥治疗组.控制(CK),功能性微生物添加处理(F),Mn2+酶诱导剂(Mn),和Mn2酶诱导剂与功能性微生物添加处理(FMn)结合进行了堆肥测试。锰(II)偶联的微生物改善了木质素的降解:FMn>Mn>F>CK。它们将木质素损失率从25.54%增加到42.61%。漆酶活性从3.45增加到43.74U/g,锰过氧化物酶活性从145.52增加到264.91U/g。基因丰度增加。微生物群落结构和优势属发生了变化。结构方程支持这样的观点,即功能性微生物与锰耦合可以改变物理化学指标,从而调节基因表达和增强酶活性。此外,真菌生长的刺激和细胞外漆酶和锰过氧化物酶活性的增加可以影响木质素的降解。本研究为堆肥产品的高效木质素降解和高效资源化利用提供了新的见解和理论支持。
The intricate structure of lignin in straw makes it challenging to hydrolyze, making it a key focus of current research. However, there has been limited study on the effect of enzyme inducer (MnSO4) combined with functional microorganisms on lignin degradation during straw composting. Based on this, four composting treatment groups were set up in this study. Control (CK), functional microorganism addition treatment (F), Mn2+ enzyme inducer (Mn), and Mn2+ enzyme inducer coupled with functional microorganism addition treatment (FMn) were tested for composting. Manganese(II)-coupled microorganisms improved lignin degradation: FMn > Mn > F > CK. They increased the lignin loss rate from 25.54 % to 42.61 %. Laccase activity increased from 3.45 to 43.74 U/g and manganese peroxidase activity increased from 145.52 to 264.91 U/g. And gene abundance was increased. Microbial community structure and dominant genera changed. Structural equations support the idea that functional microorganisms coupled with manganese can modify physicochemical indices, thereby regulating gene expression and enhancing enzyme activity. Furthermore, the stimulation of fungal growth and increased extracellular laccase and manganese peroxidase activities can affect the degradation of lignin. This study provides new insights and theoretical support for efficient lignin degradation and efficient resource utilization of compost products.