重金属被认为是工业废水中最常见的污染物。在30种细菌分离物中,只有3个分离株对Zn2,Fe2,Pb2,Co2,Mn2,Ni2和Cd2的金属电阻活性最高。生化与DNA同源性鉴定具有99.58%的相似性,99.79%,99.86%的分离株被鉴定并保存在WDCM中,分别,例如,柯贝肠杆菌OM144907SCUF0000311、阴沟肠杆菌OM180597SCUF0000312和火龙肠杆菌OM181067SCUF0000313。对Ni2,Fe2和Mn2的重金属浓度对E.kobei和E.cloacae的最小耐受活性(MIC)分别为25、15和15mmol/l,对于Zn2,Pb2,Co2和Cd2,则为10mmol/l,而对于Hormaechei,Ni2,Fe2和Mn2为15mmol/l,Zn2,Pb2,Co2和Cd2为10mmol/l。联合体和单独应用细菌分离物,以100%去除重金属,200%,进行了300%的工业废水浓度,结果表明,从未浓缩的污染样品(100%)中,Zn2,Fe2,Pb2,Mn2,Ni2和Cd2的去除率超过90%。由三个菌株报告。随着污染样品浓度加倍(200%),阴沟肠杆菌对Zn+2、Pb+2、Mn+2、Ni+2和Cd+2的去除效率最高,为70。75、66、65和57%,分别。将污染样品浓度提高到300%后的去除效率表明,阴沟肠杆菌去除除Pb2以外的所有测试重金属的45%以上。最终,阴沟肠杆菌暴露效率最高,建议在较高浓度下去除重金属。
Heavy metals are considered the most common pollutants in industrial wastewater areas. Out of thirty bacterial isolates, only 3 isolates sighted the highest metal resistance activity for Zn+2, Fe+2, Pb+2, Co+2, Mn+2, Ni+2, and Cd+2. The biochemical and DNA homology identification with similarities 99.58%, 99.79%, and 99.86% of those isolates was identified and deposited in WDCM, respectively, as Enterobacter kobei OM144907 SCUF0000311, Enterobacter cloacae OM180597 SCUF0000312, and Enterobacter hormaechei OM181067 SCUF0000313. The minimum tolerance activity (MIC) of heavy metal concentrations against E. kobei and E. cloacae was 25, 15, and 15 mmol/l for Ni+2, Fe+2, and Mn+2, respectively, and 10 mmol/l for Zn+2, Pb+2, Co+2, and Cd+2, while against E. hormaechei, it is 15 mmol/l for Ni+2, Fe+2, and Mn+2 and 10 mmol/l for Zn+2, Pb+2, Co+2, and Cd+2. The consortium and solitary application of bacterial isolates towards heavy metal removal at 100%, 200%, and 300% industrial wastewater concentrations were conducted and showed that more than 90% removal of Zn+2, Fe+2, Pb+2, Mn+2, Ni+2, and Cd+2 from a non-concentrated polluted sample (100%) was reported by the three strains. With doubling the polluted sample concentration (200%), the highest removal efficiency for Zn+2, Pb+2, Mn+2, Ni+2, and Cd+2 was reported by E. cloacae as 70. 75, 66, 65, and 57%, respectively. Removal efficiency after increasing the polluted sample concentration to 300% showed that E. cloacae removed above 45% of all tested heavy metals except Pb+2. Ultimately, E. cloacae exposed the highest efficiency with recommendations for heavy metals removal under higher concentrations.