■肝硬化中常见肠道菌群失调和细胞因子异常。本研究旨在评估它们之间的相关性。
■在肝硬化患者和对照组的血浆中,使用多重测定检查27种细胞因子。检查了亚硝酸盐(内皮功能障碍生物标志物一氧化氮的稳定代谢物)和脂多糖(LPS)的血浆水平。通过16SrRNA基因测序评估粪便微生物群。
■IL-1b水平,IL-2,IL-6,IL-13,IP-10,IFN-g,TNF-a,LPS,肝硬化患者的亚硝酸盐含量高于对照组,而IL-4,IL-7和PDGF-BB的水平较低。LPS水平与IL-1b水平直接相关,IL1-Ra,IL-9,IL-17,PDGF-BB,IL-6,TNF-a,和亚硝酸盐。亚硝酸盐水平与TNF-α水平显著直接相关,GM-CSF,IL-17和IL-12与IL-7水平呈负相关。TNF-a水平与腹水严重程度和淋巴结的丰度直接相关,肠杆菌科,Veillonellaceae,和克雷伯菌属,虽然与Firmicutes的丰度成反比,梭菌,和下颗粒。IFN-g水平与拟杆菌科的丰度直接相关,乳酸杆菌科,拟杆菌,和Megasphaera,与Verrucomicrobiota的丰度成反比,Akkermansiaceae,科氏杆菌科,Akkermansia,Collinsella,还有Gemella.IL-1b水平与Comamonadaceae和肠杆菌科的丰度直接相关,与Marinifilaceae和Dialister的丰度成反比。IL-6水平与肠杆菌科细菌的丰度直接相关,肝性脑病,和腹水的严重程度,与肽链球菌科的丰度呈负相关,链球菌科,和链球菌。
■有害肠道微生物群分类群和内毒素血症的丰度与促炎细胞因子的水平直接相关。
UNASSIGNED: Gut dysbiosis and abnormal cytokine profiles are common in cirrhosis. This study aimed to evaluate the correlations between them.
UNASSIGNED: In the blood plasma of cirrhosis patients and controls, 27 cytokines were examined using a multiplex assay. The plasma levels of nitrites (stable metabolites of the endothelial dysfunction biomarker nitric oxide) and lipopolysaccharide (LPS) were examined. The fecal microbiota was assessed by 16S rRNA gene sequencing.
UNASSIGNED: Levels of IL-1b, IL-2, IL-6, IL-13, IP-10, IFN-g, TNF-a, LPS, and nitrites were higher in cirrhosis patients than in controls, while levels of IL-4, IL-7, and PDGF-BB were lower. The LPS level was directly correlated with the levels of IL-1b, IL1-Ra, IL-9, IL-17, PDGF-BB, IL-6, TNF-a, and nitrites. The nitrite level was significantly directly correlated with the levels of TNF-a, GM-CSF, IL-17, and IL-12, and inversely correlated with the IL-7 level. TNF-a levels were directly correlated with ascites severity and the abundance of Negativicutes, Enterobacteriaceae, Veillonellaceae, and Klebsiella, while inversely correlated with the abundance of Firmicutes, Clostridia, and Subdoligranulum. IFN-g levels were directly correlated with the abundance of Bacteroidaceae, Lactobacillaceae, Bacteroides, and Megasphaera, and inversely correlated with the abundance of Verrucomicrobiota, Akkermansiaceae, Coriobacteriaceae, Akkermansia, Collinsella, and Gemella. IL-1b levels were directly correlated with the abundance of Comamonadaceae and Enterobacteriaceae and inversely correlated with the abundance of Marinifilaceae and Dialister. IL-6 levels were directly correlated with the abundance of Enterobacteriaceae, hepatic encephalopathy, and ascites severity, and inversely correlated with the abundance of Peptostreptococcaceae, Streptococcaceae, and Streptococcus.
UNASSIGNED: The abundance of harmful gut microbiota taxa and endotoxinemia directly correlates with the levels of proinflammatory cytokines.