光调控对三种根内真菌分离株生长和产酶的影响,即假雌蕊(EF13),镰刀菌(EF5),和小木霉(PH22),已确定。分离株暴露于蓝色,红色,绿色,黄色,白色荧光灯(12h光-12h暗光周期)(测试),和24小时黑暗(对照)条件。结果表明,交替的明暗条件导致大多数真菌分离物中形成暗环,但在PH22中却不存在。红光诱导孢子形成,而黄光在所有分离物中引起较高的生物量(0.19±0.01g,0.07±0.00g,和0.11±0.00g,分别对于EF13,PH22和EF5)与在黑暗中孵育相比。结果还表明,蓝光诱导所有分离株中PH22的淀粉酶活性(15.31±0.45U/mL)和L-天冬酰胺酶活性(0.45±0.01U/mL,0.55±0.39U/mL,和0.38±0.01U/mL,分别对于EF13,PH22和EF5)与两个对照条件进行比较。绿光增强了木聚糖酶的产生(6.57±0.42U/mL,10.64±0.12U/mL,EF13,PH22和EF5分别为7.55±0.56U/mL)和纤维素酶(6.49±0.48U/mL,9.57±0.25U/mL,和7.28±0.63U/mL,分别用于EF13、PH22和EF5)。相比之下,红光是最不有效的光处理,因为酶的产生最少,淀粉酶水平较低,纤维素酶,木聚糖酶,和检测到L-天冬酰胺酶。最后,所有三种根内真菌都是光反应性的,用红光和黄光调节真菌生长,以及通过蓝光和绿光操纵酶的生产。
The influence of light regulation on the growth and enzyme production of three endolichenic fungal isolates, i.e. Pseudopestalotiopsis theae (EF13), Fusarium solani (EF5), and Xylaria venustula (PH22), was determined. The isolates were exposed to blue, red, green, yellow, white fluorescent light (12 h light-12 h dark photoperiod) (test), and 24 h dark (control) conditions. Results revealed that the alternating light-dark conditions resulted in the formation of dark rings in most fungal isolates but was absent in PH22. Red light induced sporulation while yellow light elicited higher biomass in all isolates (0.19 ± 0.01 g, 0.07 ± 0.00 g, and 0.11 ± 0.00 g, for EF13, PH22, and EF5, respectively) as compared to incubation in the dark. Results also showed that blue light induced higher amylase activity in PH22 (15.31 ± 0.45 U/mL) and L-asparaginase activity in all isolates (0.45 ± 0.01 U/mL, 0.55 ± 0.39 U/mL, and 0.38 ± 0.01 U/mL, for EF13, PH22, and EF5, respectively) compared to both control conditions. Green light enhanced the production of xylanase (6.57 ± 0.42 U/mL, 10.64 ± 0.12 U/mL, and 7.55 ± 0.56 U/mL for EF13, PH22, and EF5, respectively) and cellulase (6.49 ± 0.48 U/mL, 9.57 ± 0.25 U/mL, and 7.28 ± 0.63 U/mL, for EF13, PH22, and EF5, respectively). In contrast, red light was the least effective light treatment as production of enzymes was the least, with lower levels of amylase, cellulase, xylanase, and L-asparaginase detected. To conclude, all three endolichenic fungi are light-responsive, with fungal growth regulated with the use of red light and yellow light, and manipulation of enzyme production via blue and green light.