Intermittent hydroxylamine (NH2OH) dosing strategy was applied to enhance the stability of partial nitrification and total nitrogen (N) removal efficiency (TNRE) in a continuous-flow process. The results showed 2 mg/L of NH2OH dosing (once every 6 h) could maintain stably partial nitrification with nitrite accumulation rate (NAR) of 91.6 % and TNRE of 92.6 %. The typical cycle suggested NH2OH dosing could promote simultaneous nitrification-denitrification (SND) and endogenous denitrification (END) while inhibit exogenous denitrification (EXD). Nitrification characteristics indicated the NH2OH dosing enhanced stability of partial nitrification by suppressing specific nitrite oxidation rate (SNOR), Nitrospira and nitrite oxidoreductase enzyme (Nxr). The microbial community suggested the aerobic denitrfiers, denitrifying glycogen accumulating organisms (DGAOs) and traditional denitrfiers were the potential contributor for advanced N removal. Moreover, NH2OH dosage was positively associated with NAR, SND and END. Overall, this study offers a feasible strategy to maintain sustainably partial nitrification that has great application potential.

This study investigated the nutrient removal and microbial community succession in moving bed biofilm reactor under stable and three levels of influent carbon/nitrogen (C/N) ratio fluctuation (± 10%, ± 20%, and ± 30%). Under the conditions of influent C/N ratio fluctuation, the removal efficiency of COD and PO43--P decreased 4.7-6.4% and 3.7-12.9%, respectively, while the nitrogen removal was almost unaffected. A sharp decrease in the content of culturable functional bacteria related to nitrogen and phosphorus removal including nitrite-oxidizing bacteria (NOB), aerobic denitrifying bacteria (DNB), and polyphosphate-accumulating organisms (PAOs) from the carrier biofilm was observed. Sequencing analysis revealed that the abundance of Candidatus Competibacter increased 10.3-25.9% and became the dominant genus responsible for denitrification, potentially indicating that nitrate was removed via endogenous denitrification under the influent C/N ratio fluctuation. The above results will provide basic data for the nutrient removal in decentralized wastewater treatment under highly variable influent conditions.

The creation of large amounts of excess sludge and residual nitrogen are critical issues in wastewater biotreatment. This study introduced Fe(II) into an oligotrophic anaerobic reactor (OARFe) that was implemented to modify an anoxic-oxic process to motivate in-situ sludge reduction and enhance denitrification under an effective electron shuttle among organic matter, nitrogen, and Fe. The addition of 15 mg L-1 Fe(II) resulted in a sludge reduction efficiency reached 32.0% with a decreased effluent nitrate concentration of 33.3%. This was mostly attributed to the electron transfer from Fe(II) to organic matters and nitrogen species in OARFe. The participation of Fe(II) led to the upregulation of Geothrix and Terrimonas, which caused active organic matter hydrolysis and cell lysis to stimulate the release of extracellular polymeric substances (EPS) and substance transfer between each layer of EPS. The higher utilization of released bioavailable dissolved organic matter improved endogenous denitrification, which can be combined with iron autotrophic denitrification to realize multiple electron donor-based nitrogen removal pathways, resulting in an increased nitrate removal rate of 58.2% in the absence of external carbon sources. These functional bacteria associated with the transformation of nitrogen and carbon and cycling between ferrous and ferric ions were enriched in OARFe, which contributed to efficient electron transport occurred both inside and outside the cell and increased 2,3,5-triphenyltetrazolium chloride electronic transport system activity by 46.9%. This contributed to the potential operational costs of chemical addition and sludge disposal of Fe-AO being 1.9 times lower than those of conventional A2O processes. These results imply that the addition of ferrous ions to an oligotrophic anaerobic zone for wastewater treatment has the potential for low-cost pollution control.

Removing nitrogen and phosphorus from low ratio of chemical oxygen demand to total nitrogen and temperature municipal wastewater stays a challenge. In this study, a pilot-scale anaerobic/aerobic/anoxic sequencing batch reactor (A/O/A-SBR) system first treated 15 m3/d actual municipal wastewater at 8.1-26.4 °C for 224 days. At the temperature of 15.7 °C, total nitrogen in influent and effluent were 45.5 and 10.9 mg/L, and phosphorus in influent and effluent were 3.9 and 0.1 mg/L. 16 s RNA sequencing results showed the relative abundance of Competibacter and Tetrasphaera raised to 1.25 % and 1.52 %. The strategy of excessive, no and normal sludge discharge enriched and balanced the functional bacteria, achieving an endogenous denitrification ratio more than 43.3 %. Sludge reduction and short aerobic time were beneficial to energy saving contrast with a Beijing municipal wastewater treatment. This study has significant implications for the practical application of the AOA-SBR process.

Realizing the quick enrichment and development of denitrifying phosphorus accumulating organisms (DPAOs) in actual household wastewater and industrial nitrate wastewater has significant research significance. In this study, a novel operation mode of anaerobic-oxic-anoxic (AOA) was adopted to successfully realize the enrichment and cultivation of DPAOs in urban domestic wastewater. Adjusting influent COD to PO43--P ratio, shortening the aerobic time and decreasing the aeration volume were conducive to select DPAOs in microbial populations. The system was operated for 180 days and the DPAOs were well enriched during the stable operation with the percentage of Dechloromonas increased to 5.1 %. Accordingly, the effluent PO43--P was < 0.3 mg P/L, the removal efficiency of phosphorus was 96.9 % and the removal efficiency of nitrate was 92.5 %. Above all, DPR can be successfully applied to AOA systems with good phosphorus removal performance.

Continuous flow processes for the in situ determination of N2O emissions during low C/N municipal wastewater treatment have rarely been reported. The anaerobic/aerobic/anoxic (AOA) process has recently shown promising potential in energy savings and advanced nitrogen removal, but it still needs to be comprehensively explored in relation to N2O emissions for its carbon reduction advantages. In this study, a novel gas-collecting continuous flow reactor was designed to comprehensively evaluate the emissions of N2O from the gas and liquid phases of the AOA process. Additionally, the measures of enhancing endogenous denitrification (ED) and self-enriching anaerobic ammonium oxidation (Anammox) were employed to optimize nitrogen removal and achieve N2O reduction in the anoxic zone. The results showed that enhanced ED coupled with Anammox led to an increase in the nitrogen removal efficiency (NRE) from 67.65 to 81.96%, an enhancement of the NO3- removal rate from 1.76 mgN/(L h) to 3.99 mgN/(L h), and the N2O emission factor in the anoxic zone decreased from 0.28 to 0.06%. Impressively, ED eliminated 91.46 ± 2.47% of the dissolved N2O from the upstream aerobic zone, and the dissolved N2O in the effluent was reduced to less than 0.01 mg/L. This study provides valuable strategies for fully evaluating N2O emissions and N2O reduction from the AOA process.

Achieving economic and efficient removal of nutrients in mainstream wastewater treatment plants (WWTPs) continues to be a challenging research topic. In this study, a continuous-flow anaerobic/aerobic/anoxic system with sludge double recirculation (AOA-SDR), which integrated partial nitrification (PN), endogenous denitrification (ED) and nitrite-type denitrifying phosphorus removal (nDNPR), was constructed to treat real carbon-limited municipal wastewater. The average effluent concentrations of total inorganic nitrogen (TIN) and PO43--P during the stable operation period were 1.8 and 0.3 mg/L, respectively. PN was achieved with an average nitrite accumulation ratio of 90.4 % by combined strategies. Adequate storage of polyhydroxyalkanoates and glycogen in the anaerobic zone promoted the subsequent nitrogen removal capacity. In the anoxic zone, nitrite served as the main electron acceptor for the denitrifying phosphorus removal process. Mass balance analysis revealed that nDNPR contributed to 23.6 % of TIN removal and 44.7 % of PO43--P removal. The enrichment of Nitrosomonas (0.45 %) and Ellin 6067 (1.31 %), along with the washout of Nitrospira (0.15 %) provided the bacterial basis for the successful implementation of PN. Other dominant endogenous heterotrophic bacteria, such as Dechlormonas (10.81 %) and Candidatus Accumulibacter (2.96 %), ensured simultaneous nitrogen and phosphorus removal performance. The successful validation of integrating PN, ED and nDNPR for advanced nutrient removal in the AOA-SDR process provides a transformative technology for WWTPs.

Applications of post-denitrification processes are subjected to low reaction rates caused by a lack of carbon resources. To offer a solution for reaction rate promotion, this research found a pilot-scale anaerobic/aerobic/anoxic bioreactor treating 55-120 m3/d low-strength municipal wastewater for 273 days. A short hydraulic retention time (HRT, 5-6 h) and a high nitrogen removal rate (63.2 ± 9.3 g-N/m3·d) were achieved using HRT optimization. The effluent total nitrogen concentration was maintained at 5.8 ± 1.4 mg/L while operating at a high nitrogen loading rate of 86.2 ± 12.8 g-N/m3·d. The short aeration (1.25-1.5 h) minimized the Glycogen loss. The endogenous denitrification rate increased to above 1.0 mg/(g-VSS·h). The functional genus Ca. Competibacter enriched to 2.3 %, guaranteeing the efficient post-denitrification process. Dechloromonas rose to 1.1 %, aiding in the synchronous phosphorus removal. These findings offered fresh insights into AOA processes to achieve energy/cost-saving wastewater treatment.

Simultaneous bio-treatment processes of organic carbon (C)-, nitrogen (N)-, and phosphorus (P)-containing wastewater are challenged by insufficient carbon sources in the effluent. In the present study, two parallel anaerobic/aerobic sequencing batch reactors (R-1 and R-2) treating low C/N (≤4) wastewater were employed using different partial nitrification start-up strategies, controlled reduced aeration, and decreased sludge retention time. Advanced removal efficiencies for NH4+-N (≥96%), total nitrogen (TN, ≥86%), PO43--P (≥95%), and CODintra (≥91%) were realized, with TN and PO43--P effluent concentrations of 10.0 ± 3.5 and 0.11 ± 0.3 mg/L in R-1 and 9.28 ± 4.0 and 0.11 ± 0.1 mg/L in R-2, respectively. Higher nitrite accumulation rate (nearly 100%) and TN (121.1 ± 0.7 mg TN/g VSS·d) and P (12.5 ± 0.6 mg PO43--P/g VSS·d) removal loadings were obtained in R-2 by a thorough elimination of nitrite-oxidizing bacteria. Moreover, different microbial structures and nutrient removal pathways were identified. Denitrifying glycogen-accumulating organisms (Candidatus Competibacter) and phosphorus-accumulating organisms (PAOs) (Tetrasphaera) removed N and P with partial nitrification-endogenous denitrification pathways and aerobic P removal in R-1. In R-2, aerobic denitrifying bacteria (Psychrobacter) and PAOs ensured N and P removal through the partial nitrification-aerobic denitrification and aerobic P removal pathways. Compared to R-1, R-2 offers greater efficiency, convenience, and scope to further reduce carbon-source demand.

Abstract:This paper investigated the effect of nitrogen loading rates (NLRs) on stability of aerobic granular sludge (AGS) for treating simulated ionic rare earth mine wastewater with high ammonia nitrogen and extremely low organic content. Mature AGS from a sequencing batch reactor (SBR) was seeded into five identical SBRs (R1, R2, R3, R4 and R5). The five reactors were operated with different NLRs (0.2, 0.4, 0.8, 1.2 and 1.6 kg/m3·d). After 30 days of operation, R1, R2 and R5 were dominated by broken granules, while most of the granules in R3 and R4 still maintained a complete structure. The properties of granules from R1, R2, R3, R4 and R5 deteriorated to varying degrees, while the granules from R3 and R4 showed better stability than that from R1, R2 and R5. In R1, R2, R3 and R4, the steady-state ammonia nitrogen removal efficiencies were all greater than 90%, and the steady-state removal efficiencies of total inorganic nitrogen (TIN) were approximately 30%. In R5, the removal efficiencies of ammonia nitrogen and TIN were both approximately 70%. The dominant nitrifying and denitrifying bacterial genera of the granules from the five reactors were Nitrosomonas and Thauera, respectively, and their relative abundance was much higher in granules from R3 and R4. The results demonstrated that a relative equilibrium between the growth and metabolism of nitrifying/denitrifying bacteria was achieved when NLR was between 0.8 and 1.2 kg/m3·d, which could provide technical support for the stability maintenance of AGS in the treatment of ionic rare earth mine wastewater.