■1,2-二炔酰-sn-甘油-3-磷酸胆碱(DMPC)是一种有前途的生物活性传递系统的乳化剂,但由于缺乏具有成本效益和可扩展的合成路线,其工业应用受到限制。本研究的目的是通过替代常用的柱色谱法经济地生产高纯度DMPC,并评估乳化性能。
■DMPC由sn-甘油-3-磷酸胆碱使用Steglich酯化然后从乙酸乙酯和丙酮中连续重结晶来合成。使用各种光谱和色谱技术鉴定DMPC的结构并确认其纯度。通过检查储存对使用大豆油与(i)大豆PC制备的o/w乳液的性能的影响来评估乳化性能,(ii)大豆PC+DMPC(1:1,w/w),和(iii)作为乳化剂的DMPC。
■去除了DMPC合成过程中形成的化学杂质,最终纯度为96%,熔融转变温度为37.6℃。三种乳液(大豆PC,大豆PC+DMPC,和DMPC)在两周的储存期间观察到,DMPC型乳液比大豆PC型乳液更稳定,在6个月内显示较小的粒度分布。
■通过经济的方法合成了高纯度的DMPC,基于DMPC的乳液表现出物理化学稳定,强调其在食品和制药行业相关应用中的潜力。我们的发现表明,DMPC有望作为乳化剂在食品工业中具有广泛的应用。
UNASSIGNED: 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) is a promising emulsifier for bioactive delivery systems, but its industrial applications are limited by the lack of cost-effective and scalable synthetic routes. The purpose of this study was to economically produce high-purity DMPC by replacing commonly used column chromatography methods and to evaluate the emulsifying performance.
UNASSIGNED: DMPC was synthesized from sn-glycero-3-phosphocholine using Steglich esterification followed by sequential recrystallization from ethyl acetate and acetone. The structure of DMPC was identified and its purity was confirmed using various spectroscopy and chromatography techniques. The emulsifying performance was evaluated by examining the effects of storage on the properties of o/w emulsions prepared using soybean oil with (i) soy PC, (ii) soy PC + DMPC (1:1, w/w), and (iii) DMPC as emulsifiers.
UNASSIGNED: The chemical impurities formed during the synthesis of DMPC was removed, and its final purity was 96%, and the melt transition temperature was 37.6°C. No visible difference between the three emulsions (soy PC, soy PC+DMPC, and DMPC) was observed during two-week storage, and the DMPC-based emulsion was more stable than soy PC emulsion, showing smaller particle size distribution during 6 months.
UNASSIGNED: The highly pure DMPC was synthesized by an economical method, and DMPC-based emulsions demonstrated physicochemical stable, highlighting its potential for food and pharmaceutical industry-related applications. Our findings suggest that DMPC holds promise as an emulsifier with broad applications in the food industry.