在现代家禽业中,具有抗氧化潜力的新型植物性生物活性化合物的应用旨在提高生产率和质量,并最大限度地减少相关疾病的压力。在这里,杨梅素,一种天然类黄酮,首次对肉鸡的表现进行了评估,抗氧化剂和免疫调节功能,对付禽类球虫病.总共500只一天大的雏鸡被分为五组。阴性(NC)和感染对照组(IC)饲喂不含添加剂的对照饮食,后者感染了艾美球虫。补充有杨梅素(Myc)的组饲喂Myc的对照饮食(各200、400和600mg/kg饮食)。在第14天,除NC以外的所有雏鸡都受到混合艾美球虫卵囊的攻击。在饲喂600mg/kg的组中检测到总体生长速率和饲料转化率的显着改善,与IC组不同。值得注意的是,饲喂400和600mg/kg的组显示出较高的总肉类抗氧化能力,而氧化和脂质过氧化生物标志物(过氧化氢:H2O2;活性氧:ROS;丙二醛:MDA)的减少。值得注意的是,谷胱甘肽过氧化物酶的上调;GSH-Px,过氧化氢酶;CAT,超氧化物歧化酶;SOD,血红素加氧酶-1;空肠和肌肉中的HO-1和NAD(P)H脱氢酶醌1NQO1基因随着补充Myc水平的增加而明显观察到。在21dpi,混合艾美耳球虫引起的球菌性病变的严重程度(p<0.05)。在喂食600mg/kgMyc的组中,卵囊排泄大大减少。在IC组中,血清C反应蛋白水平升高;CRP和一氧化氮;NO和炎症生物标志物的上调表达(白细胞介素-1β;IL-1β,白细胞介素-6;白细胞介素-6,肿瘤坏死因子-α;肿瘤坏死因子-α,趋化细胞因子;CCL20,基质细胞衍生因子-1;CXCL13和鸟类防御素;AvBD612)在Myc喂养组中较高水平消退。一起来看,这些发现表明Myc在调节免疫反应和减少与球虫挑战相关的生长抑制方面具有良好的抗氧化作用.
In the modern poultry industry, the application of novel phytogenic bioactive compounds with antioxidant potential aims to enhance productivity and quality and to minimize the stress of associated diseases. Herein, myricetin, a natural flavonoid, was evaluated for the first time on broiler chickens\' performance, antioxidants and immune modulating functions, and tackling avian coccidiosis. A total of 500 one-day-old chicks were divided into five groups. The negative (NC) and infected control (IC) groups were fed a control diet without additives, and the latter was infected with Eimeria spp. Groups supplemented with myricetin (Myc) were fed a control diet of Myc (200, 400 and 600 mg/kg diet each). On d 14, all chicks except those in NC were challenged with oocysts of mixed Eimeria spp. Significant improvements in the overall growth rate and feed conversion ratio were detected in the group that was fed 600 mg/kg, unlike the IC group. Notably, groups that were fed 400 and 600 mg/kg showed higher total meat antioxidant capacity with an inverse reduction in oxidative and lipid peroxidation biomarkers (hydrogen peroxide: H2O2; reactive oxygen species: ROS; Malondialdehyde: MDA). Of note, the upregulation of glutathione peroxidase; GSH-Px, catalase; CAT, superoxide dismutase; SOD, heme oxygenase-1; HO-1 and NAD(P)H dehydrogenase quinone 1 NQO1 genes in jejunum and muscle were prominently observed with increasing levels of supplemental Myc. At 21 dpi, the severity of coccoidal lesions (p < 0.05) induced by mixed Eimeria spp. and oocyst excretion were greatly reduced in the group that was fed 600 mg/kg of Myc. In the IC group, higher serum levels of C-reactive protein; CRP and nitric oxide; and NO and the upregulated expression of inflammatory biomarkers (interleukin-1β; IL-1β, interleukin-6; IL-6, tumor necrosis factor-α; TNF-α, chemotactic cytokines; CCL20, stromal cell-derived factor-1; CXCL13, and avian defensins; AvBD612) were subsided in higher levels in the Myc-fed groups. Taken together, these findings indicate the promising antioxidant role of Myc in modulating immune responses and reducing growth depression associated with coccidia challenges.