大叶球虫是家兔常见的病原菌,导致嗜睡,减肥,腹泻,甚至在严重病例感染后死亡。目前预防兔球虫病的方法是在饮食中添加抗球虫药物。然而,有许多关于耐药性和药物残留的担忧。在我们的研究中,克隆并表达rEmMIC2和rEmMIC3蛋白,以评估作为重组亚单位疫苗候选抗原的潜力.通过相对增重比评估rEmMIC2和rEmMIC3的保护作用,卵囊减少率,抗球虫指数,饲料转化率,病理改变,临床症状,特异性IgG抗体,和兔子的细胞因子水平。rEmMIC2和rEmMIC3的分子量分别为18.69kDa和17.47kDa,分别。在球虫挑战之后,对照组表现为厌食症和软便,而实验组几乎没有厌食症症状。与对照组明显不同,免疫rEmMIC2和rEmMIC3组的相对增重率分别为78.37%和75.29%,分别,卵囊减少分别为77.95%和76.09%,分别,抗球虫指数分别为171.12和169.29。IgG抗体,IFN-γ,实验组IL-4、IL-10和IL-17水平显著升高。结果表明,rEmMIC2和rEmMIC3具有作为疫苗候选抗原的潜力。
Eimeria magna is a common pathogen in rabbits, which results in lethargy, weight loss, diarrhea, and even death in severe cases after infection. The current method for preventing rabbit coccidiosis is to add anticoccidial drugs to the diet. However, there are many concerns about drug resistance and drug residues. In our study, the rEmMIC2 and rEmMIC3 proteins were cloned and expressed to evaluate potential as recombinant subunit vaccine candidate antigens. The protective effects of rEmMIC2 and rEmMIC3 were evaluated by the relative weight gain ratio, oocyst decrease rate, anticoccidial index, feed conversion ratio, pathological alterations, clinical symptoms, specific IgG antibody, and cytokine levels in rabbits. The molecular weights of rEmMIC2 and rEmMIC3 were 18.69 kDa and 17.47 kDa, respectively. After the coccidia challenge, the control groups showed anorexia and soft poop, whereas the experimental group showed few anorexia symptoms. Significantly different from the control group, the relative weight gain ratios of the immunized rEmMIC2 and rEmMIC3 groups were 78.37% and 75.29%, respectively, and the oocyst reduction was 77.95% and 76.09%, respectively, and the anticoccidial index was 171.12 and 169.29, respectively. IgG antibody, IFN-γ, IL-4, IL-10, and IL-17 levels were significantly increased in the experimental group. The results showed that rEmMIC2 and rEmMIC3 have potential as vaccine candidate antigens.