粘藻莫利特氏菌(M.粘虫)和海虱(Lepeophthheirussalmonis)是主要感染大西洋鲑鱼(Salmosalar)皮肤的严重病原体,这对养殖业造成了重大的经济损失。然而,宿主在转录后水平的免疫防御的发病机制和分子机制仍不清楚。可变剪接(AS)是一种进化上保守的转录后机制,可以大大提高转录组和蛋白质组的丰富度。在这项研究中,在粘胶分枝杆菌和海虱感染后,来自大西洋鲑鱼皮肤组织的转录组数据用于检查AS谱及其差异表达模式。总的来说,我们在对照(CON)组中确定了33,044个AS事件(涉及13,718个基因),粘胶分枝杆菌感染(MV)组中的35,147个AS事件(涉及14,340个基因),海虱感染(LC)组发生30,364例AS事件(涉及13,142个基因)。在我们研究中确定的五种类型的AS(即跳过外显子(SE),替代5'剪接位点(A5SS),替代3'剪接位点(A3SS),互斥外显子(MXE),和保留的内含子(RI)),SE是所有三组中最普遍的类型(即CON,MV,和LC组)。在MV和LC感染的情况下,在SE事件中观察到剪接(PSI)水平降低,提示MV或LC感染会升高外显子跳跃同种型,并促进许多DAS基因中较短转录本的选择。此外,大多数差异AS基因与mRNA调节相关的途径有关,上皮或肌肉发育,和免疫反应。这些发现为AS在鱼类中宿主-病原体相互作用中的作用提供了新的见解,并代表了AS对鱼类细菌和寄生虫感染的反应的首次比较分析。
Moritella viscosa (M. viscosa) and sea lice (Lepeophtheirus salmonis) are severe pathogens that primarily infect the skin of Atlantic salmon (Salmo salar), which cause significant economic losses in the farming industry. However, the pathogenesis and molecular mechanisms underlying the host\'s immune defence at the post-transcriptional level remain unclear. Alternative splicing (AS) is an evolutionarily conserved post-transcriptional mechanism that can greatly increase the richness of the transcriptome and proteome. In this study, transcriptomic data derived from skin tissues of Atlantic salmon after M. viscosa and sea lice infections were used to examine the AS profiles and their differential expression patterns. In total, we identified 33,044 AS events (involving 13,718 genes) in the control (CON) group, 35,147 AS events (involving 14,340 genes) in the M. viscosa infection (MV) group, and 30,364 AS events (involving 13,142 genes) in the sea lice infection (LC) group, respectively. Among the five types of AS identified in our study (i.e., SE, A5SS, A3SS, MXE, and RI), SE was the most prevalent type in all three groups (i.e., CON, MV, and LC groups). Decreased percent-spliced-in (PSI) levels were observed in SE events under both MV- and LC-infected conditions, suggesting that MV or LC infection elevated exon-skipping isoforms and promoted the selection of shorter transcripts in numerous DAS genes. In addition, most of the differential AS genes were found to be associated with pathways related to mRNA regulation, epithelial or muscle development, and immune response. These findings provide novel insights into the role of AS in host-pathogen interactions and represent the first comparative analysis of AS in response to bacterial and parasitic infections in fish.