土壤微生物是高寒生态系统元素循环和养分供应的重要组成部分。然而,在高山湿地退化的背景下,微生物群落组成和网络的发展尚不清楚。我们应用高通量16SrRNA基因扩增子测序来跟踪典型高山湿地(W)沿降解梯度的微生物群落变化,到湿草地(WM),到典型的草地(M),到草原(G),以及青藏高原若尔盖高山湿地地区的沙漠(D)。随着湿地退化的进行,土壤含水量(SWC)降低(W和D土壤中的79.4%和9.3%,分别)。总有机碳(TOC)总氮(TN),WM土壤中的总磷(TP)增加,然后随着高寒湿地从WM到M土壤的退化而减少,G,D,分别。湿地退化不会影响从W土到WM的微生物群落丰富度和多样性,M,和G土壤,但确实影响了D土壤的丰富度和多样性。湿地退化对微生物群落结构的影响很大,主要是由于SWC的变化,TOC,TN,和TP。SWC是影响微生物群落组成和网络的主要土壤理化性质。在湿地退化地区,放线菌,酸杆菌,Cholorflexi,和Proteovacteria在微生物网络中紧密相互作用。与W的土壤相比,WM,M,放线菌在G和D土壤的微生物共生网络中起着重要作用。这项研究有助于我们了解不同高山湿地降解过程中微生物群落组成和网络如何随土壤性质的变化而变化。
Soil microbes are important components in element cycling and nutrient supply for the development of alpine ecosystems. However, the development of microbial community compositions and networks in the context of alpine wetland degradation is unclear. We applied high-throughput 16S rRNA gene amplicon sequencing to track changes in microbial communities along degradation gradients from typical alpine wetland (W), to wet meadow (WM), to typical meadow (M), to grassland (G), and to desert (D) in the Zoige alpine wetland region on the Tibetan Plateau. Soil water content (SWC) decreased as wetland degradation progressed (79.4 and 9.3% in W and D soils, respectively). Total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP) increased in the soils of WM, and then decreased with alpine wetlands degradation from WM to the soils of M, G, and D, respectively. Wetland degradation did not affect microbial community richness and diversity from W soils to WM, M, and G soils, but did affect richness and diversity in D soils. Microbial community structure was strongly affected by wetland degradation, mainly due to changes in SWC, TOC, TN, and TP. SWC was the primary soil physicochemical property influencing microbial community compositions and networks. In wetland degradation areas, Actinobacteriota, Acidobacteriota, Cholorflexi, and Proteovacteria closely interacted in the microbial network. Compared to soils of W, WM, and M, Actinobacteriota played an important role in the microbial co-occurrence network of the G and D soils. This research contributes to our understanding of how microbial community composition and networks change with varied soil properties during degradation of different alpine wetlands.