荒漠化和盐胁迫是全球陆地生态系统丧失的主要原因。还有戈壁,代表中国内陆的盐胁迫区,对其周围环境的生态系统和生物多样性有重大影响。恢复戈壁滩是控制其扩展的重要途径,但是关于修复效果评价的研究很少。在这项研究中,不同恢复方案下的土壤,即,恢复区域的土壤(R1、R2),半还原区域(SR1,SR2),和未恢复的控制区(C1,C2),用于研究微生物多样性和理化性质的差异。结果表明,土壤主要以4-63μm(26.45-37.94%)和>63μm(57.95-72.87%)的颗粒为主。在不同的恢复级别,土壤pH值(7.96-8.43)基本不变,盐度从9.23-2.26下降到0.24-0.25,含水量保持恒定(10.98-12.27%),除了一个恢复的样品中较高(22.32%)。有效的Al,Cu,土壤中的锌增加了,但只是轻微的。总有机质(TOM)从3.86-5.20%下降到1.31-1.47%,总有机氮(TON)从0.03-0.06%下降到0.01-0.02%,但总有机碳(TOC)差异不显著。高通量检测显示,修复区细菌种群以A4b为主(6.33-9.18%),MND1(4.94-7.39%),和Vicinamibacteraceae(7.04-7.39%)。关于古细菌,修复区的样本主要是第二类海洋(76.17-81.49%)和念珠菌(6.07-9.75%)。PCoA显示不同的恢复水平是样品间差异的主要原因。此外,盐度是导致这种差异的主要因素,但是它被这些功能属中的一些的恢复和定向富集所抑制。因此,沙漠恢复应侧重于节约用水,而不是增加养分。种植耐盐和耐旱的植被将有助于沙漠的初步恢复和土壤微生物含量的恢复,因为土壤随着时间的推移而迁移,创建元素的循环。恢复通过有益微生物刺激和增强土壤的微生物多样性。
Desertification and salt stress are major causes of terrestrial ecosystem loss worldwide, and the Gobi, representing a salt-stressed area in inland China, has a major impact on the ecosystems and biodiversity of its surrounding environment. The restoration of the Gobi Desert is an important way to control its expansion, but there are few studies on the evaluation of restoration. In this study, soils under different restoration scenarios, namely, soils in restored areas (R1, R2), semi-restored areas (SR1, SR2), and unrestored control areas (C1, C2), were used to investigate differences in microbial diversity and physicochemical properties. The results showed that the soil was mainly dominated by particles of 4-63 μm (26.45-37.94%) and >63 μm (57.95-72.87%). Across the different restoration levels, the soil pH (7.96-8.43) remained basically unchanged, salinity decreased from 9.23-2.26 to 0.24-0.25, and water content remained constant (10.98-12.27%) except for one restored sample in which it was higher (22.32%). The effective Al, Cu, and Zn in the soil increased, but only slightly. Total organic matter (TOM) decreased from 3.86-5.20% to 1.31-1.47%, and total organic nitrogen (TON) decreased from 0.03-0.06% to 0.01-0.02%, but the difference in total organic carbon (TOC) was not significant. High-throughput testing revealed that the bacterial population of the restored area was dominated by A4b (6.33-9.18%), MND1 (4.94-7.39%), and Vicinamibacteraceae (7.04-7.39%). Regarding archaea, samples from the restored areas were dominated by Marine Group II (76.17-81.49%) and Candidatus Nitrososphaera (6.07-9.75%). PCoA showed that the different restoration levels were the main cause of the differences between the samples. Additionally, salinity was the dominant factor that induced this difference, but it was inhibited by the restoration and targeted enrichment of some of these functional genera. Desert restoration should therefore focus on conserving water rather than adding nutrients. Planting salt- and drought-tolerant vegetation will contribute to the initial restoration of the desert and the restoration of the microbiological content of the soil as it migrates over time, creating a cycle of elements. Restoration stimulates and enhances the microbial diversity of the soil via beneficial microorganisms.