大黄素是一种天然存在的蒽醌衍生物,具有广泛的药理活性,包括神经保护和抗炎活性。我们的目的是评估大黄素对肝细胞癌(HCC)在大鼠模型中使用增殖的抗癌活性,入侵,和血管生成生物标志物。肝癌诱导后,评估肝损伤和肝切片的组织病理学。氧化应激生物标志物的mRNA和蛋白质的肝脏表达,HO-1,Nrf2;有丝分裂激活生物标志物,ERK5,PKCδ;组织破坏生物标志物,ADAMTS4;组织稳态生物标志物,聚集物;细胞纤溶生物标志物,MMP3;和细胞血管生成生物标志物,测量VEGF。与HCC组相比,大黄素增加了生存率并减少了肝结节的数量。此外,大黄素降低了所有PKC的mRNA和蛋白质的表达升高,ERK5、ADAMTS4、MMP3、VEGF与HCC组比拟。另一方面,与HCC组相比,大黄素增加了Nrf2,HO-1和聚集蛋白聚糖的mRNA和蛋白的表达。因此,大黄素是一种有前途的抗癌药物,可以预防肝癌的预后和浸润。它通过许多作用机制起作用,如阻断氧化应激,扩散,入侵,和血管生成。
Emodin is a naturally occurring anthraquinone derivative with a wide range of pharmacological activities, including neuroprotective and anti-inflammatory activities. We aim to assess the anticancer activity of emodin against hepatocellular carcinoma (HCC) in rat models using the proliferation, invasion, and angiogenesis biomarkers. After induction of HCC, assessment of the liver impairment and the histopathology of liver sections were investigated. Hepatic expression of both mRNA and protein of the oxidative stress biomarkers, HO-1, Nrf2; the mitogenic activation biomarkers,
ERK5, PKCδ; the tissue destruction biomarker, ADAMTS4; the tissue homeostasis biomarker, aggregan; the cellular fibrinolytic biomarker, MMP3; and of the cellular angiogenesis biomarker, VEGF were measured. Emodin increased the survival percentage and reduced the number of hepatic nodules compared to the HCC group. Besides, emodin reduced the elevated expression of both mRNA and proteins of all PKC,
ERK5, ADAMTS4, MMP3, and VEGF compared with the HCC group. On the other hand, emodin increased the expression of mRNA and proteins of Nrf2, HO-1, and aggrecan compared with the HCC group. Therefore, emodin is a promising anticancer agent against HCC preventing the cancer prognosis and infiltration. It works through many mechanisms of action, such as blocking oxidative stress, proliferation, invasion, and angiogenesis.