背景:肺癌(LC)患者的生活质量(QoL)较差,容易患有心理疾病。以前的研究较少关注遗传因素与QoL之间的关系,抑郁症,LC患者的焦虑状态。本研究旨在探讨ERCC1和ERCC2的SNP和单倍型与QoL之间的关系。LC患者的抑郁和焦虑状态。
方法:QoL,使用欧洲癌症研究与治疗组织(EORTC)核心生活质量问卷(QLQ-C30)评估291例LC患者的抑郁和焦虑状态,EORTC生活质量问卷-肺癌13(QLQ-LC13),SDS和SAS。使用改进的多重连接检测反应(iMLDR)技术检测ERCC1和ERCC2的9个标签SNP。使用软件Haploview4.2进行单倍型分析。通过回归分析分析LC患者的SNP或单倍型与QoL或抑郁或焦虑之间的关联。
结果:ERCC1rs11615与情绪功能相关(P=0.027),ERCC1rs3212986与焦虑评分相关(P=0.018)。ERCC1rs762562-rs3212986单倍型与认知功能相关(P=0.029),躯体功能(P=0.014)和吞咽困难(OR=3.32,P=0.044)。ERCC1rs3212986-rs11615AG单倍型患者的认知功能(调整后的Beta=-5.42)和躯体功能(调整后的Beta=-6.55)较差,并且有更严重的食欲不振(调整后的OR=1.67)和吞咽困难(调整后的OR=4.43)(所有调整后P<0.05)。ERCC2rs13181-rs3916874-rs238416单倍型与情绪功能相关(P=0.035),其他部位疼痛(OR1.88,P=0.014),胸痛(OR0.42,P=0.02),吞咽困难(OR2.82,P=0.048),焦虑状态(OR0.23,P=0.009)。
结论:在对环境因素进行调整后,ERCC1和ERCC2的SNP和单倍型与QoL的不同域相关,LC患者的抑郁和焦虑。
BACKGROUND: Patients with lung cancer (LC) have a poor quality of life (QoL) and easily suffer from psychological diseases. Previous studies focused less on the relationship between genetic factors and QoL, depression, and anxiety status in LC patients. The current study is intended to explore the relationship between SNPs and haplotypes of ERCC1 and
ERCC2 and the QoL, depression and anxiety status of patients with LC.
METHODS: QoL, depression and anxiety status were assessed in 291 LC patients using the European Organization for Research and Treatment of Cancer (EORTC) Core Quality of Life Questionnaire (QLQ-C30), EORTC Quality of Life Questionnaire-Lung Cancer 13 (QLQ-LC13), SDS and SAS. Nine tag SNPs of ERCC1 and
ERCC2 were detected using an improved multiplex ligation detection reaction (iMLDR) technique. Haplotype analysis was conducted using the software Haploview 4.2. The association between SNPs or haplotypes and QoL or depression or anxiety in LC patients was analyzed by regression analysis.
RESULTS: ERCC1 rs11615 was associated with emotional functioning (P = 0.027), and ERCC1 rs3212986 was associated with anxiety scores (P = 0.018). ERCC1 rs762562-rs3212986 haplotype was associated with cognitive function (P = 0.029), somatic function (P = 0.014) and dysphagia (OR = 3.32, P = 0.044). Patients with ERCC1 rs3212986-rs11615 AG haplotype had worse cognitive function (adjusted Beta = - 5.42) and somatic function (adjusted Beta = - 6.55) and had severer symptoms of loss of appetite (adjusted OR = 1.67) and dysphagia (adjusted OR = 4.43) (All adjusted P < 0.05).
ERCC2 rs13181-rs3916874-rs238416 haplotype was associated with emotional functioning (P = 0.035), pain at other sites (OR 1.88, P = 0.014), chest pain (OR 0.42, P = 0.02), dysphagia (OR 2.82, P = 0.048), and anxiety status (OR 0.23, P = 0.009).
CONCLUSIONS: After adjustment for environmental factors, SNPs and haplotypes of ERCC1 and
ERCC2 were associated with different domains of QoL, depression and anxiety in LC patients.