据推测,手术引发的炎症反应可能会诱导促进增殖的分子的释放,存活癌细胞的侵袭和转移。为了检验这个假设,分析结直肠癌手术患者多种炎症相关循环因子水平。LuminexxMAP系统用于同时评估IL-1β水平,IL-1ra,IL-2、IL-4、IL-5、IL-6、IL-7、IL-8、IL-9、IL-10、IL-12、IL-13、IL-15、IL-17、FGF、eotaxin,G-CSF,GM-CSF,IFN-γ,IP-10,MCP-1,MIP-1α,MIP-1β,PDGF-BB,RANTES,20例大肠癌患者和10例年龄匹配的非肿瘤患者的TNF-α和VEGF。在癌症患者中,在基线(手术前)和腹腔镜手术后的不同时间点(最多30天)进行分析。IL-1β水平显著升高,IL-7,IL-8,G-CSF,与基线时的对照组相比,在结直肠癌患者中检测到IFN-γ和TNF-α。在结直肠癌患者中,循环水平在手术后和手术后第30天逐渐下降,与对照组相比不再不同.这些发现表明,与对照组相比,结直肠癌患者中几种细胞因子的表达水平更高,并且在腹腔镜癌症手术后未观察到几种炎症相关循环因子的显着增加。需要在不同和更大的患者队列中进行确认和验证。
It has been hypothesized that inflammatory response triggered by surgery might induce the release of molecules that could promote proliferation, invasion and metastasis of surviving cancer cells. To test this hypothesis, the levels of multiple inflammation-related circulating factors were analyzed in patients undergoing surgery for colorectal cancer. A Luminex xMAP system was used to simultaneously assess levels of IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, FGF, eotaxin, G-CSF, GM-CSF, IFN-γ, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF-α and VEGF in 20 colorectal cancer patients and 10 age-matched non-neoplastic patients. In cancer patients analyses were performed at baseline (before surgery) and at different time points (up to 30 days) following laparoscopic surgery. Significantly higher levels of IL-1β, IL-7, IL-8, G-CSF, IFN-γ and TNF-α were detected in colorectal cancer patients compared to controls at baseline. In colorectal cancer patients, circulating levels decreased progressively following surgery and after day 30 post-surgery were no longer different from controls. These findings suggest that expression levels of several cytokines are higher in colorectal cancer patients compared to control subjects and no significant increase in several inflammation-related circulating factors is observed following laparoscopic surgery for cancer. Confirmation and validation in a different and larger cohort of patients are warranted.