Avian infectious bronchitis (AIB) is a highly transmissible infection that affects the poultry industry globally. This study aims to isolate and characterize emerging strains of infectious bronchitis virus (IBV) from field samples of layer chickens in Bangladesh. A total of 108 samples (trachea, lung, and kidney) were taken from dead and sick layer chickens from 18 farms in 4 areas detecting outbreaks in Bangladesh. The samples were processed and inoculated in embryonated chicken eggs (ECEs) and finally screened by the trypsin-induced hemagglutination (THA) test. Using various techniques such as hemagglutination inhibition (HI), agar gel immuno-diffusion (AGID), virus neutralization test (VNT), reverse transcription-polymerase chain reaction (RT-PCR), and nucleotide sequencing, we were able to identify and confirm the isolated IBV viruses. The study also determined the hemagglutination (HA) pattern of isolated virus using avian and mammalian red blood cells. The pathogenicity of the isolated IBV was determined using embryonated chicken eggs and day-old chicks. The study found that 8 samples were positive for IBV using ECEs, and 4 were positive by the THA test. These isolates were confirmed using HI, AGID, and VN tests. S1 gene-based RT-PCR confirmed all four isolates as IBV, with the recent isolates belonging to the genotype-QX and being similar to IBV isolates from Thailand, Saudi Arabia, and India. The HA pattern of the recent isolates showed that the isolated IBV was virulent. The pathogenicity test also revealed that the four isolates were highly pathogenic. The study indicated that the prevalent genotype (QX) of the IBV strain is present in the layer chicken population of Bangladesh.

The impacts of extreme coastal events (ECEs), such as marine inundations or extreme wind events, on lake ecosystems vary widely from minimal to catastrophic. Accurately predicting the response of a specific system remains challenging due to a limited understanding of the attributes that drive the resilience of lakes. In an attempt to better understand the possible impacts of ECEs on shallow brackish lakes, we employed a paleolimnological approach to reconstruct the responses of Broad Pond to four ECEs identifiable from clear sedimentary markers and dated to ca. 1740, 1790, 1862, and 1993 CE. We aimed to evaluate the overall impacts of ECEs on Broad Pond and their specific effects on various hydrobiont groups. We reinvestigated a previously studied sediment core covering the last ca. 400 years by examining terrestrial and aquatic pollen, spores and non-pollen palynomorphs, cladocerans, and chironomids. Additionally, available diatom data were reexamined. Overall, Broad Pond exhibited resilient responses to ECEs, as indicated by mostly limited compositional turnovers in our proxy records. Statistically significant relationships between species composition and ECEs were observed only for diatoms. The only prolonged change identified is the spread of previously near-absent chironomids in the aftermath of a slight lake refreshment likely associated with the ca. 1740 CE event. This event was also followed by a short-lived (ca. 15 years) five-fold increase in the abundance of Scenedesmus that could have been triggered by the effects of the ECE on nutrient availability. The impact of the remaining three ECEs was discernible only in the diatom record, consistently showing a decline in two calcifobic and oligotrophic species, Achnanthidium petersenii and Platessa oblongella, also evident for the ca. 1740 CE event. The relatively minor ecosystem alteration induced by ECEs in Broad Pond lacks a single explanation and requires consideration of multiple site- and event-specific factors.