尽管杂交可能会使分类实践复杂化,它可以是动物物种之间的共同点。动物杂交不仅可以帮助在自然界中产生表型和物种多样性,而且还要了解实验室中表型进化的遗传和基因组基础。我们使用线粒体CO1和来自双消化限制性位点相关DNA测序(ddRADseq)文库的核基因座,评估了两个Hercules甲虫物种之间圈养的F1杂种的遗传组成。我们表明,F1杂种与母本物种的样本进行了遗传聚类,D.Grantii,基于CO1数据。核基因组数据,另一方面,清楚地表明F1个体在基因上介于D.maya之间,父系物种,还有D.Grantii,基于主成分分析。我们的结果还表明,采样设计可能对使用ddRADseq数据集推断的遗传结构和杂种个体产生重大影响。我们从了解种内和种间表型差异和融合的起源和维持方面,讨论了研究这种杂种后代基因组学的重要性和潜力。
Although hybridization may complicate taxonomic practices, it can be common between animal species. Animal hybridization not only can help with generating phenotypic and species diversity in nature, but also with understanding the genetic and genomic basis of phenotypic evolution in the laboratory. We assessed the genetic composition of captive bred F1 hybrids between two Hercules beetle species using mitochondrial CO1 and nuclear loci from a double-digest restriction-site associated DNA sequencing (ddRADseq) library. We showed that the F1 hybrids were genetically clustered with samples from the maternal species, D. grantii, based on CO1 data. Nuclear genome data, on the other hand, clearly showed that the F1 individuals were genetically intermediate between D. maya, the paternal species, and D. grantii, based on a principal component analysis. Our results also revealed that sampling design may have a major impact on the inferred genetic structure and hybrid individuals using ddRADseq data sets. We discuss the importance and potential from studying the genomics of this hybrid progeny in terms of understanding the origin and maintenance of both intraspecific and interspecific phenotypic divergence and convergence.