目的:本研究的主要目的是评估角膜描记器非侵入性破裂时间(NIBUT)测量的可重复性,两个或三个部分测量,并推荐适合实践的方法。另一个目标是验证重复测量不会影响测量值。
方法:38名健康志愿者(30名女性和8名男性),年龄在19至50岁之间。只测量每个志愿者的一只眼睛。该研究被设计为前瞻性研究。每个受试者适应实验室的当地条件15分钟,随后进行了两个系列的NIBUT测量(测试,重新测试)在Oculus3角膜描记器上。两个系列之间的最小时间间隔为10分钟,其中每个系列包含三个部分测量大约3三个测量在给定的系列。重复性通过Bland-Altman分析进行评估,并表示为重复性系数。在任何情况下,仅监测泪膜第一次破裂的时间。
结果:统计学分析在单个系列中NIBUT的部分测量值(p=0.92,p=0.81)和当比较所有6个测量值(p=0.95)时,均未显示统计学上显著的差异。部分测量的平均值范围为13.6s至14.4s。重复性系数为15.0s,方法A的12.1s和10.0s,B和C,分别。对低NIBUT(<10s)的12只眼的补充分析显示,该组的可重复性在统计学上显着提高,系数为7.0s(方法A),6.0s(B)和4.6s(C)。
结论:从三个连续测量(具有理想的几分钟的足够间隔)中确定NIBUT显着提高了可重复性。这种重复的NIBUT测量对测量值没有显著影响。可以推荐用于在角膜描记器上测量NIBUT的上述方法在实践中使用。
OBJECTIVE: The primary aim of this study is to evaluate the repeatability of noninvasive break-up time (NIBUT) measurement by keratograph when it is determined from one, two or three partial measurements, and to recommend a suitable methodology for practice. Another goal is to verify that repeated measurements do not affect the measured value.
METHODS: Thirty-eight healthy volunteers (30 women and 8 men) aged between 19 and 50 years old were included in the study, in which only one eye of each volunteer was measured. The study was designed as a prospective one. Each subject adapted to the local conditions of the laboratory for 15 minutes and subsequently underwent two series of NIBUT measurements (test, retest) on an OCULUS 3 Keratograph. The minimum time interval between the two series was 10 minutes, in which each series contained three partial measurements approximately 3 three measurements in the given series. Repeatability was assessed by a Bland-Altman analysis and expressed as a repeatability coefficient. In every case, only the time of the first break-up of the tear film was monitored.
RESULTS: The statistical analysis did not show statistically significant differences both between partial measurements of NIBUT in the individual series (p = 0.92, p = 0.81) and when comparing all six measurements (p = 0.95). The mean values of the partial measurements ranged from 13.6 s to 14.4 s. The repeatability coefficients were found to be 15.0 s, 12.1 s and 10.0 s for methodologies A, B and C, respectively. A supplementary analysis for 12 eyes with low NIBUT (< 10 s) showed statistically significantly better repeatability in this group, with coefficients of 7.0 s (methodology A), 6.0 s (B) and 4.6 s (C).
CONCLUSIONS: Determination of NIBUT from three consecutive measurements (with a sufficient interval of ideally a few minutes) significantly improves repeatability. Such repeated NIBUT measurements do not have a significant effect on the measured value. The mentioned methodology for measuring NIBUT on a keratograph can be recommended for use in practice.