目的:目前的阿片类药物检测实验室方法包括免疫测定法的初步筛选,可提供有效但非特异性的结果,以及随后的液相色谱-串联质谱(LC-MS/MS)确认,可提供准确的结果,但需要大量的样品制备和周转时间。实时直接分析(DART)串联质谱被评估为具有有效样品制备和周转时间的准确阿片样物质检测的替代方法。
方法:DART-MS/MS通过测试不同温度的方法进行了优化,操作模式,提取方法,水解时间,和漩涡时间。通过测试分析测量范围,该方法对12种阿片类药物进行了评估,百分比结转,精度研究,稳定性,与LC-MS/MS的方法比较
结果:DART-MS/MS对6-乙酰吗啡的检测显示出高灵敏度和特异性,可待因,氢吗啡酮,羟吗啡酮,氢可酮,纳洛酮,丁丙诺啡,降芬太尼,还有尿样中的芬太尼.然而,它的性能对于去甲丁丙诺啡来说是次优的,吗啡和羟考酮.
结论:在这项概念验证研究中,评估DART-MS/MS对尿液中阿片类药物的快速定量确定性测试。需要进一步的研究将其应用扩展到药物测试的其他领域。
OBJECTIVE: Current laboratory methods for opioid detection involve an initial screening with immunoassays which offers efficient but non-specific results and a subsequent liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmation which offers accurate results but requires extensive sample preparation and turnaround time. Direct Analysis in Real Time (DART) tandem mass spectrometry is evaluated as an alternative approach for accurate opioid detection with efficient sample preparation and turnaround time.
METHODS: DART-MS/MS was optimized by testing the method with varying temperatures, operation modes, extraction methods, hydrolysis times, and vortex times. The method was evaluated for 12 opioids by testing the analytical measurement range, percent carryover, precision studies, stability, and method-to-method comparison with LC-MS/MS.
RESULTS: DART-MS/MS shows high sensitivity and specificity for the detection of 6-acetylmorphine, codeine, hydromorphone, oxymorphone, hydrocodone, naloxone, buprenorphine, norfentanyl, and fentanyl in urine samples. However, its performance was suboptimal for norbuprenorphine, morphine and oxycodone.
CONCLUSIONS: In this proof-of-concept study, DART-MS/MS is evaluated for its rapid quantitative definitive testing of opioids drugs in urine. Further research is needed to expand its application to other areas of drug testing.