罗克布。例如G.Don在东南亚广泛存在。据报道,其油树脂已用于生物柴油生产。两种不同的生物柴油生产工艺产生树脂副产品,即脱胶(DG)和蒸馏(DT)。气相色谱-质谱法鉴定了油树脂中的倍半萜和三萜,DG,和DT;和油树脂中的长链烃。高效液相色谱法检测到双萜卡波为标记化合物,在DG中检测到最高到最低的量,DT,和含油树脂,分别。油树脂,DG,DT比双蝶卡波发挥更大的细胞毒性,还有Melphalan,化疗药物.油树脂,DG,DT在T细胞白血病(Jurkat)中具有不同程度的细胞毒性,宫颈腺癌(HeLa),和人肝细胞癌(HepG2)细胞,而与非癌症Vero细胞相比,在Jurkat细胞中发现了最高的选择性。Diptercarpol在HepG2细胞中表现出最高的细胞毒性,在Jurkat细胞中表现出最低的细胞毒性。油树脂,DG,DT诱导Jurkat细胞凋亡。在含油树脂中,DG,和DT,diptercarpol和其他化合物可以协同作用,导致细胞毒性和细胞凋亡诱导作用。油树脂,DG,DT可能是抗癌药物的潜在来源。Diptercarpol可以作为生物标志物,用于跟踪来自D.alatus的样品的抗癌活性。
Dipterocarpus alatus Roxb. ex G. Don is widely found in Southeast Asia. Its oleo-resin has reportedly been used in biodiesel production. Two different biodiesel production processes produce resinous byproducts, namely degumming (DG) and distillation (DT). Gas chromatography-mass spectrometry identified sesquiterpenes and triterpenes in oleo-resin, DG, and DT; and long-chain hydrocarbons in oleo-resin. High-performance liquid chromatography detected
dipterocarpol as a marker compound, with the highest to lowest amounts detected in DG, DT, and oleo-resin, respectively. Oleo-resin, DG, and DT exerted more cytotoxicity than
dipterocarpol, and melphalan, a chemotherapeutic drug. Oleo-resin, DG, and DT exerted cytotoxicity to a different degree in T cell leukemia (Jurkat), cervical adenocarcinoma (HeLa), and human hepatocellular carcinoma (HepG2) cells, while the highest selectivity was found in the Jurkat cells compared to the non-cancer Vero cells.
Dipterocarpol exhibited the highest cytotoxicity in HepG2 cells and the lowest cytotoxicity in Jurkat cells. Oleo-resin, DG, and DT induced apoptosis in Jurkat cells. In oleo-resin, DG, and DT,
dipterocarpol and other compounds may act in synergy leading to cytotoxicity and an apoptosis-inducing effect. Oleo-resin, DG, and DT could be potential sources for anticancer agents.
Dipterocarpol could serve as a biomarker for follow ups on the anticancer activity of a sample from D. alatus.