未经批准:由于底栖微生物生产二甲基磺丙酸酯(DMSP),盐沼是有机硫化合物循环的热点,大型藻类,和盐沼植被。DMSP的降解是二甲基硫醚(DMS)的来源,形成二次有机气溶胶的重要前兆。降解DMS的微生物在控制可用于排放到大气中的DMS的量中发挥作用。先前的工作暗示沉积物微生物种群是DMS的主要汇。这里,我们展示了Sporobolusanglicus(以前称为Spartinaanglica),一种分布广泛的盐沼植物,被DMS降解微生物定殖。
UNASSIGNED:通过气相色谱和13C-DMS稳定同位素探测评估了二甲基硫化物的降解潜力,通过16SrRNA基因扩增子的高通量测序评估了叶际和根际样品中的微生物群落多样性和功能遗传潜力,甲硫醇氧化酶基因的克隆和测序,并通过叶际微生物群落的宏基因组分析。
UNASSIGNED:从叶际和根际样品中回收的微生物群落的DMS降解潜力相似。通过13C-DMS稳定同位素探测鉴定出活性DMS降解物,并包括根际样品中与甲基phaga和其他Pisciricketsiaceae相关的种群。叶球中的DMS降解剂包括黄科和卤虫科。甲烷硫醇氧化酶(mtoX)基因在沉积物样品中的多样性,在DMS和DMSP降解过程中甲硫醇代谢的标记,类似于先前检测到的saltmarshmtoX,包括甲虫和甲虫。毛球mtoX基因与沉积物mtoX不同,不包括培养细菌的近亲。与水稻等模型植物相比,盎格鲁斯根际的微生物多样性截然不同。大豆,三叶草和拟南芥,并显示出γ变形杆菌而不是α变形杆菌的优势。
UNASSIGNED:在Sporobolusanglicus的叶际和根际中微生物DMS降解的潜力表明,盐沼中的DMS循环比以前认识到的更复杂,因此需要对地上活动如何影响DMS通量进行更详细的评估。
UNASSIGNED: Saltmarshes are hotspots of organosulfur compound cycling due to production of dimethylsulfoniopropionate (DMSP) by benthic microorganisms, macroalgae, and saltmarsh vegetation. Degradation of DMSP is a source of
dimethylsulfide (DMS), an important precursor for formation of secondary organic aerosol. Microorganisms degrading DMS play a role in controlling the amount of DMS available for emission into the atmosphere. Previous work has implicated sediment microbial populations as a major sink for DMS. Here, we show that Sporobolus anglicus (previously known as Spartina anglica), a widely distributed saltmarsh plant, is colonized by DMS-degrading microorganisms.
UNASSIGNED: Dimethylsulfide degradation potential was assessed by gas chromatography and 13C-DMS stable isotope probing, microbial community diversity and functional genetic potential in phyllosphere and rhizosphere samples was assessed by high-throughput sequencing of 16S rRNA gene amplicons, cloning and sequencing of methanethiol oxidase genes, and by metagenomic analysis of phyllosphere microbial communities.
UNASSIGNED: The DMS degradation potential of microbial communities recovered from phyllosphere and rhizosphere samples was similar. Active DMS-degraders were identified by 13C-DMS stable isotope probing and included populations related to Methylophaga and other Piscirickettsiaceae in rhizosphere samples. DMS-degraders in the phyllosphere included Xanthomonadaceae and Halothiobacillaceae. The diversity in sediment samples of the methanethiol oxidase (mtoX) gene, a marker for metabolism of methanethiol during DMS and DMSP degradation, was similar to previously detected saltmarsh mtoX, including those of Methylophaga and Methylococcaeae. Phyllosphere mtoX genes were distinct from sediment mtoX and did not include close relatives of cultivated bacteria. Microbial diversity in the phyllosphere of S. anglicus was distinct compared to those of model plants such as rice, soybean, clover and Arabidopsis and showed a dominance of Gammaproteobacteria rather than Alphaproteobacteria.
UNASSIGNED: The potential for microbial DMS degradation in the phyllosphere and rhizosphere of Sporobolus anglicus suggest that DMS cycling in saltmarshes is more complex than previously recognised and calls for a more detailed assessment of how aboveground activities affect fluxes of DMS.