网藻属的线虫是几种家养和野生有蹄类动物的寄生性支气管炎和肺炎的病原体。在野生子宫颈中已经描述了各种物种,就像马鹿身上的Cervi木偶一样,最近被描述为与Dictyocauluseckerti分开的物种。在意大利,关于野生动物双足病的信息有限,而且往往过时。在这项工作中,检查了250只马鹿是否存在木偶属物种。在意大利阿尔卑斯山的两个地区(n=104,n=146,来自Stelvio国家公园),并对回收的肺虫进行了分子表征。在Valled'Aosta和Stelvio国家公园的23和32只动物中发现了夜蛾,分别。线虫,形态学鉴定为D.cervi,分子特征(18SrDNA,ITS2和coxI)。始终如一,几乎所有标本都被发现与D.cervi有关。三个人,从两个研究地点检测到,并分配给一个未描述的Dictyocaulussp。,在以前的研究中,从马鹿和小鹿中分离出的木偶标本聚集在一起。在D.cervi和未描述的Dictyocaulussp。,新分离的线虫根据其地理起源进行系统发育聚类。这项研究揭示了意大利马鹿中存在D.cervi,和一个未确定的Dictyocaulussp。应该更深入地调查。结果表明,进一步的分析应该集中在宫颈及其肺虫的群体遗传学上,以评估它们是如何进化的。或者共同进化,在整个时间和空间,并评估向养殖动物传播的潜力。
Nematodes of the genus
Dictyocaulus are the causative agents of parasitic bronchitis and pneumonia in several domestic and wild ungulates. Various species have been described in wild cervids, as the case of
Dictyocaulus cervi in red deer, recently described as a separate species from
Dictyocaulus eckerti. In Italy, information on dictyocaulosis in wildlife is limited and often outdated. In this work, 250 red deer were examined for the presence of Dictyocaulus spp. in two areas of the Italian Alps (n = 104 from Valle d\'Aosta, n = 146 from Stelvio National Park), and the retrieved lungworms were molecularly characterized. Lungworms were identified in 23 and 32 animals from Valle d\'Aosta and Stelvio National Park, respectively. The nematodes, morphologically identified as D. cervi, were characterized molecularly (18S rDNA, ITS2, and coxI). Consistently, almost all specimens were found to be phylogenetically related to D. cervi. Three individuals, detected from both study sites and assigned to an undescribed Dictyocaulus sp., clustered with
Dictyocaulus specimens isolated from red deer and fallow deer in previous studies. Within each of D. cervi and the undescribed Dictyocaulus sp., the newly isolated nematodes phylogenetically clustered based on their geographical origin. This study revealed the presence of D. cervi in Italian red deer, and an undetermined
Dictyocaulus sp. that should be more deeply investigated. The results suggest that further analyses should be focused on population genetics of cervids and their lungworms to assess how they evolved, or co-evolved, throughout time and space and to assess the potential of transmission towards farmed animals.