背景:农药的持续使用,比如敌敌畏,是农业和家庭的常见做法。然而,它通过诱导氧化应激介导的信号传导与睾丸毒性等缺陷相关。另一方面,L-精氨酸,一氧化氮的前体,据报道具有抗氧化活性,因此可以减轻敌敌畏引起的睾丸毒性。
目标:因此,本研究旨在评估L-精氨酸治疗对敌敌畏引起的睾丸毒性的影响。
方法:40只雄性Wistar大鼠随机分为4组。对照组大鼠给予0.5mL蒸馏水,敌敌畏(DDVP)处理的大鼠通过吸入暴露于DDVP15分钟,DDVP+L-精氨酸处理的大鼠暴露于DDVP,并接受100mg/kgb.w/天,而L-精氨酸处理的大鼠接受100mg/kgb.w/天。
结果:DDVP暴露显著降低睾丸一氧化氮,相对睾丸重量,精子数量减少,生存能力,和运动性,抑制血清FSH,LH,和睾丸激素水平。这些发现与睾丸丙二醛的升高有关,TNF-α,IL-6和8OHdG水平和caspase3活性,以及GSH和超氧化物歧化酶的减少。此外,组织病理学检查,观察到DDVP减少了生精小管腔中的成熟精子细胞,并在间质空间中引起局灶性血管充血。尽管如此,L-精氨酸治疗可显着减弱DDVP诱导的生化和组织学改变。
结论:这项研究表明,L-精氨酸通过抑制氧化应激改善附睾精子变量和男性性激素来减轻睾丸毒性,炎症,和DDVP暴露大鼠的细胞凋亡。
BACKGROUND: The continuous use of pesticides, such as
dichlorvos, is a common agricultural and domestic practice. However, it is associated with shortfalls like testicular toxicity through the induction of oxidative stress-mediated signaling. On the other hand, L-arginine, a precursor of nitric oxide, has been reported to exert antioxidant activities and thus may attenuate
dichlorvos-induced testicular toxicity.
OBJECTIVE: Hence, this study was designed to evaluate the effect of L-arginine treatment on
dichlorvos-induced testicular toxicity.
METHODS: Forty male Wistar rats were randomly assigned into four equal groups. The control rats were administered 0.5 mL of distilled water,
dichlorvos- (DDVP-) treated rats were exposed to DDVP via inhalation for 15 min, DDVP + L-arginine-treated rats were exposed to DDVP and also received 100 mg/kg b.w/day, while L-arginine-treated rats received 100 mg/kg b.w/day.
RESULTS: DDVP exposure significantly reduced testicular nitric oxide, relative testicular weight, lowered sperm count, viability, and motility, and suppressed serum FSH, LH, and testosterone levels. These findings were associated with a rise in testicular malondialdehyde, TNF-α, IL-6, and 8OHdG levels and caspase 3 activities, and a reduction in GSH and superoxide dismutase. Additionally, on histopathological examination, DDVP was observed to reduce mature sperm cells in the seminiferous tubular lumen and induce focal vascular congestion in the interstitial space. Nonetheless, L-arginine treatment significantly attenuated DDVP-induced biochemical and histological alterations.
CONCLUSIONS: This study showed that L-arginine attenuated testicular toxicity by improving epididymal sperm variables and male sex hormones by suppressing oxidative stress, inflammation, and apoptosis in DDVP-exposed rats.