背景:糖尿病性溃疡是一种慢性疾病,其特征是长时间的高血糖和伤口愈合延迟,伴随着内分泌失调,炎症反应,和表皮组织的微血管损伤,需要有效的临床治疗方法。几千年来,中国古代民族药理学研究记录了Poriacocos的使用(Schw。)狼在治疗糖尿病性溃疡。最近的研究证实了Poriacocos的多种药理作用(Schw。)狼,包括其缓解高血糖和表现出抗炎的潜力,抗氧化剂,和免疫调节特性,能有效缓解糖尿病溃疡症状。此外,作为一种天然药物,Poriacocos(Schw。)Wolf在糖尿病溃疡的管理中已经证明了有希望的治疗效果和安全性,具有显著的临床价值。尽管其潜在的临床疗效和在糖尿病溃疡治疗中的应用,茯苓的主要活性成分和潜在的药理机制(Schw。)狼仍不清楚。进一步的研究对于在该领域的药物开发奠定坚实的基础至关重要。
■在这项研究中,我们的目的是确定Poriacocos的活性化合物和潜在靶标(Schw。)Wolf使用UHPLC-Q-TOF-MS和TCMSP数据库。此外,我们试图确定与糖尿病溃疡相关的目标。在富集分析之后,构建了一个蛋白质-蛋白质相互作用网络,以基于两个数据集之间的共同元素来识别hub基因。为了深入了解中心基因和活性成分的结合活性,采用分子对接分析。此外,进一步验证茯苓的治疗效果(Schw.)狼,我们使用人脐静脉血管内皮细胞和人髓性白血病单核细胞(THP-1)进行了体外实验。Poriacocos的活性成分(Schw。)沃尔夫被应用在这些实验中。我们的调查包括各种化验,如CCK-8,划痕试验,免疫荧光,西方印迹,RT-PCR,和流式细胞术,探索Poriacocos的潜力(Schw。)狼三萜提取物(PTE)治疗糖尿病溃疡。
结果:此处的发现强调了PTE是Poriacocos的主要活性成分(Schw。)狼。利用网络药理学,我们确定了与Poriacocos糖尿病性溃疡治疗相关的74个潜在目标(Schw。)狼,具有五个枢纽基因(JUN,MAPK1、STAT3、AKT1和CTNNB1)。富集分析揭示了多种途径参与治疗过程,PI3K-AKT信号通路显示显著富集。通过分子对接,我们发现该途径中的相关靶标表现出与Poriacocos活性成分的强结合(Schw。)狼。体外实验表明,PTE(10mg/L)促进人脐静脉血管内皮细胞的迁移(P<0.05)。PTE还增加了CD31和VEGFmRNA的表达(P<0.05),同时激活了p-PI3K和p-AKT的表达(P<0.05)。此外,PTE通过降低IL-1β的表达证明了其潜力,IL-6,TNF-α,和NF-κBmRNA在THP-1中的分化(P<0.05),并促进M2巨噬细胞的分化。这些结果表明PTE在治疗糖尿病性溃疡方面具有潜在的治疗作用。其通过PI3K-AKT信号通路介导的有益作用。
结论:PTE是Poriacocos的主要活性成分(Schw。)发挥治疗作用的狼。通过PI3K-AKT信号通路激活和减轻炎症反应,PTE促进血管生成,从而治愈糖尿病性溃疡。
BACKGROUND: Diabetic ulcers represent a chronic condition characterized by prolonged hyperglycemia and delayed wound healing, accompanied by endocrine disorders, inflammatory responses, and microvascular damage in the epidermal tissue, demanding effective clinical treatment approaches. For thousands of years, ancient Chinese ethnopharmacological studies have documented the use of Poria cocos (Schw.) Wolf in treating diabetic ulcers. Recent research has substantiated the diverse pharmacological effects of Poria cocos (Schw.) Wolf, including its potential to alleviate hyperglycemia and exhibit anti-inflammatory, antioxidant, and immune regulatory properties, which could effectively mitigate diabetic ulcer symptoms. Furthermore, being a natural medicine, Poria cocos (Schw.) Wolf has demonstrated promising therapeutic effects and safety in the management of diabetic ulcers, holding significant clinical value. Despite its potential clinical efficacy and applications in diabetic ulcer treatment, the primary active components and underlying pharmacological mechanisms of Poria cocos (Schw.) Wolf remains unclear. Further investigations are imperative to establish a solid foundation for drug development in this domain.
UNASSIGNED: In this study, we aimed to identify the active compounds and potential targets of Poria cocos (Schw.) Wolf using UHPLC-Q-TOF-MS and TCMSP databases. Additionally, we attempt to identify targets related to diabetic ulcers. Following enrichment analysis, a network of protein-protein interactions was constructed to identify hub genes based on the common elements between the two datasets. To gain insights into the binding activities of the hub genes and active ingredients, molecular docking analysis was employed. Furthermore, to further validate the therapeutic effect of Poria cocos (Schw.) Wolf, we exerted in vitro experiments using human umbilical vein vascular endothelial cells and human myeloid leukemia monocytes (THP-1). The active ingredient of Poria cocos (Schw.) Wolf was applied in these experiments. Our investigations included various assays, such as CCK-8, scratch test, immunofluorescence, western blotting, RT-PCR, and flow cytometry, to explore the potential of Poria cocos (Schw.) Wolf triterpenoid extract (PTE) in treating diabetic ulcers.
RESULTS: The findings here highlighted PTE as the primary active ingredient in Poria cocos (Schw.) Wolf. Utilizing network pharmacology, we identified 74 potential targets associated with diabetic ulcer treatment for Poria cocos (Schw.) Wolf, with five hub genes (JUN, MAPK1, STAT3, AKT1, and CTNNB1). Enrichment analysis revealed the involvement of multiple pathways in the therapeutic process, with the PI3K-AKT signaling pathway showing significant enrichment. Through molecular docking, we discovered that relevant targets within this pathway exhibited strong binding with the active components of Poria cocos (Schw.) Wolf. In vitro experiments unveiled that PTE (10 mg/L) facilitated the migration of human umbilical vein vascular endothelial cells (P < 0.05). PTE also increased the expression of CD31 and VEGF mRNA (P < 0.05) while activating the expressions of p-PI3K and p-AKT (P < 0.05). Moreover, PTE demonstrated its potential by reducing the expression of IL-1β, IL-6, TNF-α, and NF-κB mRNA in THP-1 (P < 0.05) and fostering M2 macrophage polarization. These results signify the potential therapeutic effects of PTE in treating diabetic ulcers, with its beneficial actions mediated through the PI3K-AKT signaling pathway.
CONCLUSIONS: PTE is the main active ingredient in Poria cocos (Schw.) Wolf that exerts therapeutic effects. Through PI3K-AKT signaling pathway activation and inflammatory response reduction, PTE promotes angiogenesis, thereby healing diabetic ulcers.