目的:本研究旨在观察白藜芦醇(RSV)对邻苯二甲酸二正丁酯(DBP)损伤的可能保护作用。大鼠附睾导管和输精管。
方法:实验使用六组大鼠:第1组:对照组;第2组:溶剂(羧甲基纤维素(CMC),10ml/kg);第3组:500mg/kg/天DBP;第4组:500mg/kg/天DBP+20mg/kg/天RSV;第5组:1000mg/kg/天DBP;第6组:1000mg/kg/天DBP+20mg/kg/天RSV。各组通过管饲法处理30天。免疫组织化学,在附睾和输精管中进行了电子显微镜和组织形态学检查。
结果:在附睾导管和输精管线粒体晶体分解中,立体纤毛的脱落和侧面的开口随DBP剂量的增加而增加,但是这些结构是用RSV回收的。DBP降低了附睾和输精管的上皮高度。在两个组织中均观察到管腔扩张。这些疾病可能导致上皮吸收功能障碍。在两种组织的TUNEL检查中,无凋亡细胞或凋亡小体。
结论:结论:DBP给药引起附睾和输精管结构变性,与剂量评估平行,RSV可以逆转这些变化,具有保护作用。
OBJECTIVE: This study aimed to observe the possible protective effects of resveratrol (RSV) against damage induced by di-n-butylphthalate (DBP), on the ductus epididymis and deferens in rats.
METHODS: Six groups of rats were used in the experiment: Group 1: Control group; Group 2: Solvent (carboxymethylcellulose (CMC), 10 ml/kg); Group 3: 500 mg/kg/day DBP; Group 4: 500 mg/kg/day DBP+20 mg/kg/day RSV; Group 5: 1000 mg/kg/day DBP; Group 6: 1000 mg/kg/day DBP + 20 mg/kg/day RSV. Groups were treated by gavage for 30 days. Immunohistochemical, electronmicroscopic and histomorphometric examinations were carried out in the epididymis and deferens.
RESULTS: In the ductus epididymis and deferens mitochondrial crystolysis, exfoliation of the stereocilia and openings in lateral surface increased with DBP dosage, but these structures were recovered with RSV. DBP reduced the epithelial height of epididymis and vas deferens. Lumen dilatation was observed in both tissues. These disorders may lead to dysfunction of epithelial absorption. In the TUNEL examinations in both tissues, there were no apoptotic cells or apoptotic bodies.
CONCLUSIONS: In conclusion, DBP administration caused structural degeneration in the epididymis and deferens, parallel to dose evaluation and RSV can reverse these changes with its protective effects.